Any feedback?
Literature summary for 3.1.1.1 extracted from
- The 'pH optimum anomaly' of intracellular enzymes of Ferroplasma acidiphilum (2006), Environ. Microbiol., 8, 416-425.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Ferroplasma acidiphilum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.45 | - |
4-nitrophenyl propionate | pH 2.0, 50°C | Ferroplasma acidiphilum |  |
| 0.47 | - |
4-nitrophenyl acetate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 0.6 | - |
4-nitrophenyl butyrate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 2.2 | - |
Triacetin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 3.5 | - |
tripropionin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 3.9 | - |
4-nitrophenyl hexanoate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 7.4 | - |
tributyrin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Ferroplasma acidiphilum | 16020 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34734 | - |
1 * 34734, calculated from sequence | Ferroplasma acidiphilum |
| 35000 | - |
non-denasturing PAGE | Ferroplasma acidiphilum |
| 36000 | - |
1 * 36000, SDS-PAGE | Ferroplasma acidiphilum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ferroplasma acidiphilum | Q2PCE5 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| purified from both Escherichia coli and Ferroplasma acidiphilum | Ferroplasma acidiphilum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butyrate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Ferroplasma acidiphilum | 4-nitrophenol + propionate | - |
? | |
| additional information | no activity with triolein | Ferroplasma acidiphilum | ? | - |
? | |
| triacetin + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
| tributyrin + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
| tripropionin + H2O | - |
Ferroplasma acidiphilum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 36000, SDS-PAGE | Ferroplasma acidiphilum |
| monomer | 1 * 34734, calculated from sequence | Ferroplasma acidiphilum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EStFa | - |
Ferroplasma acidiphilum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 1.0-2.5, hydrolysis of 4-nitrophenol propionate | Ferroplasma acidiphilum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
pH 1.7-2.0, half-life: 48 min | Ferroplasma acidiphilum |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
4-nitrophenyl hexanoate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 10 | - |
4-nitrophenyl butyrate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 23 | - |
tributyrin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 25 | - |
4-nitrophenyl acetate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 29 | - |
4-nitrophenyl propionate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 44 | - |
tripropionin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 47 | - |
Triacetin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 1.5 | - |
- |
Ferroplasma acidiphilum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1 | 3 | pH 1.0: about 70% of maximal activity, pH 3.0: about 65% of maximal activity | Ferroplasma acidiphilum |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ferroplasma acidiphilum | calculated from sequence | - |
5.91 |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.6 | - |
4-nitrophenyl hexanoate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 3.1 | - |
tributyrin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 12.7 | - |
tripropionin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 16.7 | - |
4-nitrophenyl butyrate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 21.3 | - |
Triacetin | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 53 | - |
4-nitrophenyl acetate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
| 64.2 | - |
4-nitrophenyl propionate | pH 2.0, 50°C | Ferroplasma acidiphilum | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
