Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Cereibacter sphaeroides |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.2 M MgCl2, and 0.1 M Tris, pH 8.5 | Cereibacter sphaeroides |
Protein Variants | Comment | Organism |
---|---|---|
E175K | the mutant shows 10fold reduced Mn2+ content compared to the wild type enzyme | Cereibacter sphaeroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
4-methylumbelliferyl heptanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.033 | - |
4-nitrophenyl octanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.048 | - |
4-nitrophenyl hexanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.056 | - |
4-nitrophenyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.11 | - |
paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.16 | - |
4-Methylumbelliferyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.18 | - |
1-naphthyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.22 | - |
ethyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.24 | - |
4-methylumbelliferyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.33 | - |
ethyl butyrylacetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.4 | - |
2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.42 | - |
methyl paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.42 | - |
ethyl 4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.52 | - |
4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.56 | - |
2-naphthyl propionate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.62 | - |
ethyl 2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.64 | - |
1-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.65 | - |
4-nitroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.7 | - |
isobutyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.9 | - |
1,4-diacetoxybenzene | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.2 | - |
4-methylbenzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.2 | - |
isopropyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.6 | - |
Benzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.6 | - |
4'-chloroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.8 | - |
3-acetoxypyridine | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.9 | - |
indoxyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.8 | - |
phenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | contains iron | Cereibacter sphaeroides | |
Mn2+ | contains Mn2+ | Cereibacter sphaeroides | |
Zn2+ | contains Zn2+ | Cereibacter sphaeroides |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cereibacter sphaeroides | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HisTrap HP column chromatography and Superdex 200 gel filtration | Cereibacter sphaeroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-diacetoxybenzene + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
1-naphthyl acetate + H2O | - |
Cereibacter sphaeroides | 1-naphthol + acetate | - |
? | |
1-naphthyl butyrate + H2O | - |
Cereibacter sphaeroides | 1-naphthol + butyrate | - |
? | |
2-naphthyl acetate + H2O | - |
Cereibacter sphaeroides | 2-naphthol + acetate | - |
? | |
2-naphthyl propionate + H2O | - |
Cereibacter sphaeroides | 2-naphthol + propionate | - |
? | |
2-phenethyl acetate + H2O | - |
Cereibacter sphaeroides | 2-phenylethanol + acetate | - |
? | |
2-thionaphthyl acetate + H2O | - |
Cereibacter sphaeroides | 2-thionaphthol + acetate | - |
? | |
3-acetoxypyridine + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
4'-chloroacetanilide + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
4-hydroxyphenyl acetate + H2O | - |
Cereibacter sphaeroides | 4-hydroxyphenol + acetate | - |
? | |
4-methylbenzyl acetate + H2O | - |
Cereibacter sphaeroides | 4-methylbenzyl alcohol + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferone + acetate | - |
? | |
4-methylumbelliferyl acetate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferol + acetate | - |
? | |
4-methylumbelliferyl butyrate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferone + butyrate | - |
? | |
4-methylumbelliferyl heptanoate + H2O | - |
Cereibacter sphaeroides | 4-methylumbelliferone + heptanoate | - |
? | |
4-nitroacetanilide + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
4-nitrophenyl acetate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | best substrate | Cereibacter sphaeroides | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + methylphosphonate | - |
? | |
4-nitrophenyl octanoate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + octanoate | - |
? | |
benzyl acetate + H2O | - |
Cereibacter sphaeroides | benzyl alcohol + acetate | - |
? | |
cyclohexyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
ethyl (4-nitrophenyl)acetate + H2O | - |
Cereibacter sphaeroides | ethanol + (4-nitrophenyl)acetate | - |
? | |
ethyl (naphthalen-2-yl)acetate + H2O | - |
Cereibacter sphaeroides | (naphthalen-2-yl)acetate + ethanol | - |
? | |
ethyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
ethyl butyrylacetate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
indoxyl acetate + H2O | - |
Cereibacter sphaeroides | indol-3-ol + acetate | - |
? | |
isobutyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
isobutyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | 4-nitrophenol + isobutyl methylphosphonate | - |
? | |
isopropyl 4-nitrophenyl methylphosphonate + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
methyl paraoxon + H2O | - |
Cereibacter sphaeroides | ? | - |
? | |
paraoxon + H2O | - |
Cereibacter sphaeroides | diethylphosphate + 4-nitrophenol | - |
? | |
phenyl acetate + H2O | - |
Cereibacter sphaeroides | phenol + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | - |
Cereibacter sphaeroides |
Synonyms | Comment | Organism |
---|---|---|
Rsp3690 | - |
Cereibacter sphaeroides |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
4-methylumbelliferyl heptanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.1 | - |
4'-chloroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.36 | - |
4-nitroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.5 | - |
4-nitrophenyl octanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.65 | - |
4-methylumbelliferyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.79 | - |
ethyl butyrylacetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.3 | - |
paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.7 | - |
ethyl 2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.8 | - |
ethyl 4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.1 | - |
methyl paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.3 | - |
indoxyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.5 | - |
Benzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
3.1 | - |
isopropyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
4.3 | - |
3-acetoxypyridine | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
4.8 | - |
1-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
4.9 | - |
4-Methylumbelliferyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
6.5 | - |
4-nitrophenyl hexanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
8 | - |
isobutyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
9 | - |
1-naphthyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
30 | - |
phenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
40 | - |
2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
47 | - |
4-methylbenzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
50 | - |
4-nitrophenyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
83 | - |
2-naphthyl propionate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
84 | - |
ethyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
108 | - |
1,4-diacetoxybenzene | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
120 | - |
4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
4'-chloroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.21 | - |
cyclohexyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.25 | - |
2-phenethyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.47 | - |
2-thionaphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
0.55 | - |
4-nitroacetanilide | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
1.2 | - |
indoxyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.4 | - |
3-acetoxypyridine | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.4 | - |
ethyl butyrylacetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.6 | - |
4-methylumbelliferyl heptanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.6 | - |
isopropyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.7 | - |
4-methylumbelliferyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
2.8 | - |
ethyl 2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
4.3 | - |
ethyl 4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
5 | - |
methyl paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
7.5 | - |
1-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
11 | - |
phenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
11 | - |
isobutyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
12 | - |
paraoxon | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
15 | - |
4-nitrophenyl octanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
31 | - |
4-Methylumbelliferyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
38 | - |
4-methylbenzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
100 | - |
2-naphthyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
130 | - |
4-nitrophenyl hexanoate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
130 | - |
1,4-diacetoxybenzene | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
150 | - |
2-naphthyl propionate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
160 | - |
Benzyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
230 | - |
4-nitrophenyl acetate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
380 | - |
ethyl 4-nitrophenyl methylphosphonate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
500 | - |
1-naphthyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides | |
880 | - |
4-nitrophenyl butyrate | in 2.5 mM Bicine buffer, pH 8.3, at 30°C | Cereibacter sphaeroides |