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Literature summary for 3.1.1.1 extracted from

  • Durand, N.; Carot-Sans, G.; Chertemps, T.; Bozzolan, F.; Party, V.; Renou, M.; Debernard, S.; Rosell, G.; Maibeche-Coisne, M.
    Characterization of an antennal carboxylesterase from the pest moth Spodoptera littoralis degrading a host plant odorant (2010), PLoS ONE, 5, e15026.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in sf21 cells Spodoptera littoralis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
9.6
-
 (Z)-3-hexenyl acetate Lineweaver-Burk method, in 20 mM Tris buffer (pH 7.4), at 28°C Spodoptera littoralis
11.4
-
 (Z)-3-hexenyl acetate non-linear fitting, in 20 mM Tris buffer (pH 7.4), at 28°C Spodoptera littoralis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 x * 60000, recombinant CXE10, SDS-PAGE Spodoptera littoralis
61000
-
 x * 61000, recombinant CXE10 calculated from amino acid sequence Spodoptera littoralis

Organism

Organism UniProt Comment Textmining
Spodoptera littoralis
-
 pest moth
-

Source Tissue

Source Tissue Comment Organism Textmining
antenna predominant expression in the olfactory sensilla Spodoptera littoralis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(Z)-3-hexenyl acetate + H2O nearly 100% hydrolysis (native enzyme), 89.2% hydrolysis (recombinant enzyme) Spodoptera littoralis (Z)-3-hexenol + acetate
-
 ?
(Z9,E11)-tetradec-9,11-enyl acetate + H2O about 40% hydrolysis (native enzyme), 7.63% hydrolysis (recombinant enzyme) Spodoptera littoralis (Z9,E11)-tetradec-9,11-en-1-ol + acetate
-
 ?
(Z9,E12)-tetradec-9,12-enyl acetate + H2O about 40% hydrolysis (native enzyme), 8.98% hydrolysis (recombinant enzyme) Spodoptera littoralis (Z9,E11)-tetradec-9,12-en-1-ol + acetate
-
 ?

Subunits

Subunits Comment Organism
? x * 60000, recombinant CXE10, SDS-PAGE Spodoptera littoralis
? x * 61000, recombinant CXE10 calculated from amino acid sequence Spodoptera littoralis

Synonyms

Synonyms Comment Organism
carboxyl/cholinesterase
-
 Spodoptera littoralis
CCE
-
 Spodoptera littoralis
CXE10
-
 Spodoptera littoralis

Expression

Organism Comment Expression
Spodoptera littoralis the CXE10 transcripts are highly expressed in the antennae of both sexes, with a slightly higher level in males than in female antennae (1.2fold). SlCXE10 expression is at least 15fold higher in antennae than in non-olfactory tissues. The transcript level in male antennae is strongly induced by exposure to sex pheromones and a green leaf volatile up

General Information

General Information Comment Organism
physiological function in antennae, CXE10 expression is associated with sensilla responding to the sex pheromones and to plant odours Spodoptera littoralis