Cloned (Comment) | Organism |
---|---|
CXE1 is expressed in Escherichia coli M15 cells | Malus domestica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diethyl coumarylphosphate | - |
Malus domestica | |
paraoxon | - |
Malus domestica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000053 | - |
4-methylumbelliferyl palmitate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
0.000086 | - |
4-methylumbelliferyl laurate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
0.00031 | - |
4-methylumbelliferyl octanoate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
0.000618 | - |
4-methylumbelliferyl heptanoate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
0.0227 | - |
4-Methylumbelliferyl butyrate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
0.0828 | - |
4-yethylumbelliferyl acetate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39800 | - |
x * 39800, predicted molecular mass for the N-terminal His6-tagged version of CXE1 | Malus domestica |
40000 | - |
x * 40000, SDS-PAGE | Malus domestica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Malus domestica | - |
cultivar Royal Gala AO3 | - |
Purification (Comment) | Organism |
---|---|
nickel affinity column chromatography | Malus domestica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Malus domestica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl acetate + H2O | - |
Malus domestica | 4-methylumbelliferone + acetate | - |
? | |
4-methylumbelliferyl butyrate + H2O | - |
Malus domestica | 4-methylumbelliferone + butyrate | - |
? | |
4-methylumbelliferyl heptanoate + H2O | - |
Malus domestica | 4-methylumbelliferone + heptanoate | - |
? | |
4-methylumbelliferyl laurate + H2O | best substrate | Malus domestica | 4-methylumbelliferone + laurate | - |
? | |
4-methylumbelliferyl octanoate + H2O | - |
Malus domestica | 4-methylumbelliferone + octanoate | - |
? | |
4-methylumbelliferyl palmitate + H2O | - |
Malus domestica | 4-methylumbelliferone + palmitate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Malus domestica |
? | x * 39800, predicted molecular mass for the N-terminal His6-tagged version of CXE1 | Malus domestica |
Synonyms | Comment | Organism |
---|---|---|
CXE1 | - |
Malus domestica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Malus domestica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 45 | the activity of CXE1 declines by approximately 3fold by 50°C compared to the optimum at 35°C | Malus domestica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.254 | - |
4-methylumbelliferyl palmitate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
2 | 3.7 | 4-yethylumbelliferyl acetate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
4.66 | - |
4-methylumbelliferyl octanoate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
6.38 | - |
4-methylumbelliferyl laurate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
6.94 | - |
4-methylumbelliferyl heptanoate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
69.5 | - |
4-Methylumbelliferyl butyrate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Malus domestica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | - |
Malus domestica |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000019 | - |
in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | paraoxon | |
0.0573 | - |
in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | diethyl coumarylphosphate |
Organism | Comment | Expression |
---|---|---|
Malus domestica | in wild type, gene CXE1 is expressed at low levels during early stages of fruit development, rising to a peak of expression in apple fruit at harvest maturity. It is not significantly up-regulated by ethylene in the skin of AO3 fruit. Gene CXE16 is expressed constitutively in wild type throughout fruit development, and is up-regulated by ethylene in skin of AO3 fruit for 18 h | up |
General Information | Comment | Organism |
---|---|---|
physiological function | CXE1 has an impact on apple flavour through its ability to hydrolyse relevant flavour esters in ripe apple fruit | Malus domestica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
287 | - |
4-yethylumbelliferyl acetate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
3070 | - |
4-Methylumbelliferyl butyrate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
4760 | - |
4-methylumbelliferyl palmitate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
11200 | - |
4-methylumbelliferyl heptanoate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
15100 | - |
4-methylumbelliferyl octanoate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica | |
74100 | - |
4-methylumbelliferyl laurate | in 25 mM Tris-HCl (pH 8.0), at 25°C | Malus domestica |