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Literature summary for 3.1.1.1 extracted from

  • Charbonneau, D.M.; Meddeb-Mouelhi, F.; Beauregard, M.
    A novel thermostable carboxylesterase from Geobacillus thermodenitrificans: evidence for a new carboxylesterase family (2010), J. Biochem., 148, 299-308.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli M15[pREP4] cells Geobacillus thermodenitrificans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
x * 30000, SDS-PAGE Geobacillus thermodenitrificans

Organism

Organism UniProt Comment Textmining
Geobacillus thermodenitrificans G3JWZ2
-
-
Geobacillus thermodenitrificans CMB-A2 G3JWZ2
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography, gel filtration Geobacillus thermodenitrificans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.58
-
using 4-nitrophenyl caprylate as substrate, at 50°C and pH 8.0 Geobacillus thermodenitrificans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O about 80% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O about 80% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans CMB-A2 4-nitrophenol + butyrate
-
?
4-nitrophenyl caprylate + H2O maximal activity Geobacillus thermodenitrificans 4-nitrophenol + caprylate
-
?
4-nitrophenyl caprylate + H2O maximal activity Geobacillus thermodenitrificans CMB-A2 4-nitrophenol + caprylate
-
?
4-nitrophenyl decanoate + H2O 65% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans 4-nitrophenol + decanoate
-
?
4-nitrophenyl decanoate + H2O 65% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans CMB-A2 4-nitrophenol + decanoate
-
?
4-nitrophenyl laurate + H2O 58% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans 4-nitrophenol + laurate
-
?
4-nitrophenyl myristate + H2O 36% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans 4-nitrophenol + myristate
-
?
4-nitrophenyl palmitate + H2O 12% activity compared to 4-nitrophenyl caprylate Geobacillus thermodenitrificans 4-nitrophenol + palmitate
-
?
additional information activity decreases when the chain length increases from C8 to C16, the enzyme does not show any activity on longer acyl chain triglycerides than tributyrin Geobacillus thermodenitrificans ?
-
?
additional information activity decreases when the chain length increases from C8 to C16, the enzyme does not show any activity on longer acyl chain triglycerides than tributyrin Geobacillus thermodenitrificans CMB-A2 ?
-
?
tributyrin + H2O
-
Geobacillus thermodenitrificans ?
-
?
tributyrin + H2O
-
Geobacillus thermodenitrificans CMB-A2 ?
-
?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Geobacillus thermodenitrificans

Synonyms

Synonyms Comment Organism
EstGtA2
-
Geobacillus thermodenitrificans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Geobacillus thermodenitrificans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
55 75 the enzyme retains over 90% of its maximal activity at 55 and 60°C. However, a decrease in activity is observed at 65°C (83% left), while only 15% of relative activity is observed at 75°C Geobacillus thermodenitrificans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60 80 the enzyme is stable for 42 h at 60°C, however an important decrease in stability is observed at 70°C, where the enzyme retains 63% of its initial activity, down to 43% at 75°C and further to 75% at 80°C after 10 min Geobacillus thermodenitrificans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Geobacillus thermodenitrificans

pH Range

pH Minimum pH Maximum Comment Organism
6 11 62% of relative activity at pH 6.0 and 36% at pH 11.0 Geobacillus thermodenitrificans