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Literature summary for 3.1.1.1 extracted from

  • Lee, S.
    Purification and characterisation of a carboxylesterase from a chlorpyrifos-methyl-resistant strain of Oryzaephilus surinamensis (Coleoptera: Silvanidae) (2011), Aust. J. Entomol., 50, 187-194.
No PubMed abstract available
Search for more literature for 3.1.1.1

Inhibitors

Inhibitors Comment Organism Structure
chlorpyrifos-methyl the enzyme is totally inhibited by 0.001 mM chlorpyrifos-methyl Oryzaephilus surinamensis
chlorpyrifos-methyl oxon the enzyme is totally inhibited by 0.001 mM chlorpyrifos-methyl oxon Oryzaephilus surinamensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
60000
-
 2 * 60000, SDS-PAGE Oryzaephilus surinamensis
120000
-
 gel filtration Oryzaephilus surinamensis

Organism

Organism UniProt Comment Textmining
Oryzaephilus surinamensis
-
 saw-toothed grain beetle, strains VOSCM and VOS48
-

Purification (Commentary)

Purification (Comment) Organism
Sepharose CL-6B column chromatography, DEAE Sephacel gel filtration and Sephacryl S-300 gel filtration Oryzaephilus surinamensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.77
-
 crude extract from strain VOSCM, using 4-nitrophenyl acetate as substrate, in 9.2 mM Tris-HCl buffer, pH 7.5, at 25°C Oryzaephilus surinamensis
2 2.5 enzyme from strain VOSCM after 29.2fold purification, using 4-nitrophenyl acetate as substrate, in 9.2 mM Tris-HCl buffer, pH 7.5, at 25°C Oryzaephilus surinamensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl acetate + H2O
-
 Oryzaephilus surinamensis 1-naphthol + acetate
-
 ?
2-naphthyl acetate + H2O
-
 Oryzaephilus surinamensis 2-naphthol + acetate
-
 ?
4-nitrophenyl acetate + H2O best substrate Oryzaephilus surinamensis 4-nitrophenol + acetate
-
 ?
additional information the purified enzyme does not hydrolyse insecticide substrates Oryzaephilus surinamensis ?
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 60000, SDS-PAGE Oryzaephilus surinamensis

General Information

General Information Comment Organism
physiological function carboxylesterase from the chlorpyrifos-methyl-resistant strain VOSCM of Oryzaephilus surinamensis plays a role in conferring resistance to chlorpyrifos-methyl Oryzaephilus surinamensis