Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 145% activity at 5 mM | Thalassobacillus sp. | |
additional information | enzyme activity is not affected by SDS, Tween 20 and Tween 80 (0.2% (v/v) each) | Thalassobacillus sp. | |
NaCl | optimum activity occurs at 0.5 M NaCl (w/v) with 116% activity | Thalassobacillus sp. | |
Triton X-100 | 120% activity at 0.2% (v/v) | Thalassobacillus sp. |
General Stability | Organism |
---|---|
the enzyme is quite stable up to 4 M NaCl and retains 90% of the original activity even after 12 h incubation | Thalassobacillus sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 76% residual activity at 1 mM | Thalassobacillus sp. | |
diethyl 4-nitrophenyl phosphate | complete inhibition at 5 mM | Thalassobacillus sp. | |
diethyl dicarbonate | 8% residual activity at 5 mM | Thalassobacillus sp. | |
Fe2+ | 51% residual activity at 1 mM | Thalassobacillus sp. | |
Fe3+ | 23% residual activity at 1 mM | Thalassobacillus sp. | |
additional information | not inhibited by 1 mM EDTA and beta-mercaptoethanol | Thalassobacillus sp. | |
Phenylarsine oxide | 10% residual activity at 5 mM | Thalassobacillus sp. | |
phenylmethylsulfonyl fluoride | complete inhibition at 1 mM | Thalassobacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.69 | - |
4-nitrophenyl butyrate | pH 8.5, 40°C | Thalassobacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thalassobacillus sp. | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme activity is not affected or slightly inhibited by Na+, K+, Mg2+, Ca2+, Mn2+, Co2+, Hg2+ and Zn2+ (1 mM each) | Thalassobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
1 * 45000, SDS-PAGE | Thalassobacillus sp. |
49000 | - |
analytical ultracentrifugation | Thalassobacillus sp. |
Organic Solvent | Comment | Organism |
---|---|---|
acetonitrile | 82.8% activity at 10% (v/v) | Thalassobacillus sp. |
dimethylformamide | 74% activity at 10% (v/v) | Thalassobacillus sp. |
DMSO | 55.9% activity at 10% (v/v) | Thalassobacillus sp. |
Ethanol | 75.9% activity at 10% (v/v) | Thalassobacillus sp. |
Methanol | 80.7% activity at 10% (v/v) | Thalassobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thalassobacillus sp. | - |
- |
- |
Thalassobacillus sp. DF-E4 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ultrafiltration, DEAE-Sephadex A-25 gel filtration and Sephadex G-75 gel filtration | Thalassobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
252 | - |
crude extract, using 4-nitrophenyl butyrate as substrate, at pH 8.5 and 40°C | Thalassobacillus sp. |
2046 | - |
after 8.11fold purification, using 4-nitrophenyl butyrate as substrate, at pH 8.5 and 40°C | Thalassobacillus sp. |
Storage Stability | Organism |
---|---|
4°C, 50 mM Tris-HCl buffer (pH 8.0), at least 4 weeks, the enzyme remains stable | Thalassobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl acetate + H2O | - |
Thalassobacillus sp. | 1-naphthol + acetate | - |
? | |
1-naphthyl acetate + H2O | - |
Thalassobacillus sp. DF-E4 | 1-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Thalassobacillus sp. | 2-naphthol + acetate | - |
? | |
2-naphthyl acetate + H2O | - |
Thalassobacillus sp. DF-E4 | 2-naphthol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | 89% activity compared to 4-nitrophenyl butyrate | Thalassobacillus sp. | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl acetate + H2O | 89% activity compared to 4-nitrophenyl butyrate | Thalassobacillus sp. DF-E4 | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butyrate + H2O | most efficient hydrolysis with 4-nitrophenyl butyrate | Thalassobacillus sp. | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | most efficient hydrolysis with 4-nitrophenyl butyrate | Thalassobacillus sp. DF-E4 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl decanoate + H2O | 14% activity compared to 4-nitrophenyl butyrate | Thalassobacillus sp. | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 78% activity compared to 4-nitrophenyl butyrate | Thalassobacillus sp. | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 46% activity compared to 4-nitrophenyl butyrate | Thalassobacillus sp. | 4-nitrophenol + octanoate | - |
? | |
additional information | no activity on 4-nitrophenyl ester substrates with an acyl chain length greater than C12. The enzyme has no activity towards casein or trioleoylglycerol | Thalassobacillus sp. | ? | - |
? | |
additional information | no activity on 4-nitrophenyl ester substrates with an acyl chain length greater than C12. The enzyme has no activity towards casein or trioleoylglycerol | Thalassobacillus sp. DF-E4 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Thalassobacillus sp. |
Synonyms | Comment | Organism |
---|---|---|
LIP4 | - |
Thalassobacillus sp. |
serine-type carboxylesterase | - |
Thalassobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Thalassobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | LIP4 is very active between 30 and 50°C with an optimal temperature of 40°C, and about 32 and 71% activity at 20 and 30°C, respectively | Thalassobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 80 | LIP4 is stable at temperatures below 45°C and loses about 36% of its activity when incubated for 60 min at 50°C, while the enzyme is completely inactivated at 80°C. The half-life of the enzyme is 103 min at 50°C, 36 min at 55°C, and 9 min at 60°C without any additive | Thalassobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
- |
Thalassobacillus sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 9.5 | the enzyme activity is stable at pH 6.0-9.5 for 1 h | Thalassobacillus sp. |