Cloned (Comment) | Organism |
---|---|
expressed as a fusion protein in Escherichia coli | Sulfurisphaera tokodaii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
4-nitrophenyl butyrate | - |
Sulfurisphaera tokodaii | |
0.59 | - |
4-nitrophenyl hexanoate | - |
Sulfurisphaera tokodaii | |
0.63 | - |
4-nitrophenyl octanoate | - |
Sulfurisphaera tokodaii | |
25.2 | - |
4-nitrophenyl octanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
44.7 | - |
4-nitrophenyl hexanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
127 | - |
4-nitrophenyl butanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33810 | - |
x * 33810, calculated from sequence | Sulfurisphaera tokodaii |
37000 | - |
x * 37000 (including a 3 kDa size increase by fusion tag), SDS-PAGE | Sulfurisphaera tokodaii |
Organic Solvent | Comment | Organism |
---|---|---|
1,4-dioxane | 30%, about 60% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
acetonitrile | 30%, about 50% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
dimethyl sulfoxide | 30%, anout 90% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
tert-butyl alcohol | 20%, about 90% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate | Sulfurisphaera tokodaii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfurisphaera tokodaii | Q976W8 | - |
- |
Sulfurisphaera tokodaii 7 | Q976W8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfurisphaera tokodaii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
43.2 | - |
- |
Sulfurisphaera tokodaii |
43.2 | - |
pH 7.5, 70°C | Sulfurisphaera tokodaii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl butanoate + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Sulfurisphaera tokodaii | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl butyrate + H2O | - |
Sulfurisphaera tokodaii 7 | 4-nitrophenol + butyrate | - |
? | |
4-nitrophenyl hexanoate + H2O | 31.6% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 31.5% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 31.6% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 31.5% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 16.7% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 16.7% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 16.7% of the activity with 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 16.7% of the activity compared to 4-nitrophenyl butyrate | Sulfurisphaera tokodaii 7 | 4-nitrophenol + octanoate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 33810, calculated from sequence | Sulfurisphaera tokodaii |
? | x * 37000 (including a 3 kDa size increase by fusion tag), SDS-PAGE | Sulfurisphaera tokodaii |
Synonyms | Comment | Organism |
---|---|---|
STK_00710 | locus name | Sulfurisphaera tokodaii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Sulfurisphaera tokodaii |
70 | - |
substrate: 4-nitrophenyl butyrate, 4-nitrophenyl hexanoate or 4-nitrophenyl octanoate | Sulfurisphaera tokodaii |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | 50°C: about 50% of maximal activity, 75°C: about 90% of maximal activity | Sulfurisphaera tokodaii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
pH 7.5, 2 h, completely stable | Sulfurisphaera tokodaii |
80 | - |
2 h, retains 10% of its activity | Sulfurisphaera tokodaii |
85 | - |
half-life: 40 min | Sulfurisphaera tokodaii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.2 | - |
4-nitrophenyl octanoate | - |
Sulfurisphaera tokodaii | |
25.2 | - |
4-nitrophenyl octanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
44.7 | - |
4-nitrophenyl hexanoate | - |
Sulfurisphaera tokodaii | |
44.7 | - |
4-nitrophenyl hexanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
127 | - |
4-nitrophenyl butyrate | - |
Sulfurisphaera tokodaii | |
127 | - |
4-nitrophenyl butanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Sulfurisphaera tokodaii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | pH 4.0: about 60% of maximal activity, pH 9.0: about 80% of maximal activity | Sulfurisphaera tokodaii |
4 | 9 | pH 4.0: about 55% of maximal activity, pH 9.0: about 80% of maximal activity | Sulfurisphaera tokodaii |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
25.2 | - |
4-nitrophenyl octanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
44.7 | - |
4-nitrophenyl hexanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii | |
127 | - |
4-nitrophenyl butanoate | pH 7.5, 70°C | Sulfurisphaera tokodaii |