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Literature summary for 3.1.1.1 extracted from

  • Suzuki, Y.; Miyamoto, K.; Ohta, H.
    A novel thermostable esterase from the thermoacidophilic archaeon Sulfolobus tokodaii strain 7. (2004), FEMS Microbiol. Lett., 236, 97-102.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed as a fusion protein in Escherichia coli Sulfurisphaera tokodaii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.53
-
4-nitrophenyl butyrate
-
Sulfurisphaera tokodaii
0.59
-
4-nitrophenyl hexanoate
-
Sulfurisphaera tokodaii
0.63
-
4-nitrophenyl octanoate
-
Sulfurisphaera tokodaii
25.2
-
4-nitrophenyl octanoate pH 7.5, 70°C Sulfurisphaera tokodaii
44.7
-
4-nitrophenyl hexanoate pH 7.5, 70°C Sulfurisphaera tokodaii
127
-
4-nitrophenyl butanoate pH 7.5, 70°C Sulfurisphaera tokodaii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33810
-
x * 33810, calculated from sequence Sulfurisphaera tokodaii
37000
-
x * 37000 (including a 3 kDa size increase by fusion tag), SDS-PAGE Sulfurisphaera tokodaii

Organic Solvent Stability

Organic Solvent Comment Organism
1,4-dioxane 30%, about 60% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate Sulfurisphaera tokodaii
acetonitrile 30%, about 50% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate Sulfurisphaera tokodaii
dimethyl sulfoxide 30%, anout 90% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate Sulfurisphaera tokodaii
tert-butyl alcohol 20%, about 90% inhibition, at 70°C, in 50 mM HEPES (pH 7.5) using 4-nitrophenyl butanoate as the substrate Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii Q976W8
-
-
Sulfurisphaera tokodaii 7 Q976W8
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfurisphaera tokodaii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
43.2
-
-
Sulfurisphaera tokodaii
43.2
-
pH 7.5, 70°C Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butanoate + H2O
-
Sulfurisphaera tokodaii 4-nitrophenol + butanoate
-
?
4-nitrophenyl butyrate + H2O
-
Sulfurisphaera tokodaii 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Sulfurisphaera tokodaii 7 4-nitrophenol + butyrate
-
?
4-nitrophenyl hexanoate + H2O 31.6% of the activity with 4-nitrophenyl butyrate Sulfurisphaera tokodaii 4-nitrophenol + hexanoate
-
?
4-nitrophenyl hexanoate + H2O 31.5% of the activity compared to 4-nitrophenyl butyrate Sulfurisphaera tokodaii 4-nitrophenol + hexanoate
-
?
4-nitrophenyl hexanoate + H2O 31.6% of the activity with 4-nitrophenyl butyrate Sulfurisphaera tokodaii 7 4-nitrophenol + hexanoate
-
?
4-nitrophenyl hexanoate + H2O 31.5% of the activity compared to 4-nitrophenyl butyrate Sulfurisphaera tokodaii 7 4-nitrophenol + hexanoate
-
?
4-nitrophenyl octanoate + H2O 16.7% of the activity with 4-nitrophenyl butyrate Sulfurisphaera tokodaii 4-nitrophenol + octanoate
-
?
4-nitrophenyl octanoate + H2O 16.7% of the activity compared to 4-nitrophenyl butyrate Sulfurisphaera tokodaii 4-nitrophenol + octanoate
-
?
4-nitrophenyl octanoate + H2O 16.7% of the activity with 4-nitrophenyl butyrate Sulfurisphaera tokodaii 7 4-nitrophenol + octanoate
-
?
4-nitrophenyl octanoate + H2O 16.7% of the activity compared to 4-nitrophenyl butyrate Sulfurisphaera tokodaii 7 4-nitrophenol + octanoate
-
?

Subunits

Subunits Comment Organism
? x * 33810, calculated from sequence Sulfurisphaera tokodaii
? x * 37000 (including a 3 kDa size increase by fusion tag), SDS-PAGE Sulfurisphaera tokodaii

Synonyms

Synonyms Comment Organism
STK_00710 locus name Sulfurisphaera tokodaii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
Sulfurisphaera tokodaii
70
-
substrate: 4-nitrophenyl butyrate, 4-nitrophenyl hexanoate or 4-nitrophenyl octanoate Sulfurisphaera tokodaii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
50 75 50°C: about 50% of maximal activity, 75°C: about 90% of maximal activity Sulfurisphaera tokodaii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
pH 7.5, 2 h, completely stable Sulfurisphaera tokodaii
80
-
2 h, retains 10% of its activity Sulfurisphaera tokodaii
85
-
half-life: 40 min Sulfurisphaera tokodaii

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
25.2
-
4-nitrophenyl octanoate
-
Sulfurisphaera tokodaii
25.2
-
4-nitrophenyl octanoate pH 7.5, 70°C Sulfurisphaera tokodaii
44.7
-
4-nitrophenyl hexanoate
-
Sulfurisphaera tokodaii
44.7
-
4-nitrophenyl hexanoate pH 7.5, 70°C Sulfurisphaera tokodaii
127
-
4-nitrophenyl butyrate
-
Sulfurisphaera tokodaii
127
-
4-nitrophenyl butanoate pH 7.5, 70°C Sulfurisphaera tokodaii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
Sulfurisphaera tokodaii

pH Range

pH Minimum pH Maximum Comment Organism
4 9 pH 4.0: about 60% of maximal activity, pH 9.0: about 80% of maximal activity Sulfurisphaera tokodaii
4 9 pH 4.0: about 55% of maximal activity, pH 9.0: about 80% of maximal activity Sulfurisphaera tokodaii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
25.2
-
4-nitrophenyl octanoate pH 7.5, 70°C Sulfurisphaera tokodaii
44.7
-
4-nitrophenyl hexanoate pH 7.5, 70°C Sulfurisphaera tokodaii
127
-
4-nitrophenyl butanoate pH 7.5, 70°C Sulfurisphaera tokodaii