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Literature summary for 3.1.1.1 extracted from
- A carboxylesterase from the thermoacidophilic archaeon Sulfolobus solfataricus P1; purification, characterization, and expression (2006), Biochim. Biophys. Acta, 1760, 820-828.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | enzyme retains 97% of activity after 60 min in 1 M urea. Not inhibitory: 1% Tween 20, 1% Lubrol | Saccharolobus solfataricus | |
| sodium dodecylsulfate | 1%, 60 min at 30°C, 59% residual activity | Saccharolobus solfataricus |  |
| Triton X-100 | 1%, 60 min at 30°C, 81% residual activity | Saccharolobus solfataricus | |
| Tween 80 | 1%, 60 min at 30°C, 76% residual activity | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
gel filtration | Saccharolobus solfataricus |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| 2-propanol | 40%, 60 min at 30°C, 91% residual activity | Saccharolobus solfataricus |
| Acetone | 40%, 60 min at 30°C, 61% residual activity | Saccharolobus solfataricus |
| Ethanol | 40%, 60 min at 30°C, 90% residual activity | Saccharolobus solfataricus |
| Methanol | 40%, 60 min at 30°C, 86% residual activity | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus P1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3975 | - |
pH 7.0, 50°C | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + palmitate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Saccharolobus solfataricus P1 | 4-nitrophenol + palmitate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE, MALDI-TOF | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
5 days, 41% residual activity | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Saccharolobus solfataricus |
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Release 2023.1 (January 2023)
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