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Literature summary for 3.1.1.1 extracted from

  • Sobek, H.; Görisch, H.
    Purification and characterization of a heat-stable esterase from the thermoacidophilic archaebacterium Sulfolobus acidocaldarius. (1988), Biochem. J., 250, 453-458.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour diffusion method Sulfolobus acidocaldarius

Inhibitors

Inhibitors Comment Organism Structure
eserine 10 mM, 19% inhibition Sulfolobus acidocaldarius
paraoxon 0.01 mM, complete inhibition Sulfolobus acidocaldarius
phenylmethanesulfonyl fluoride 0.1 mM, complete inhibition Sulfolobus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01036
-
4-nitrophenyl hexanoate pH 8.0, 25°C Sulfolobus acidocaldarius
0.0215
-
4-nitrophenyl acetate pH 8.0, 25°C Sulfolobus acidocaldarius
0.0338
-
4-nitrophenyl propionate pH 8.0, 25°C Sulfolobus acidocaldarius
0.0606
-
4-nitrophenyl octanoate pH 8.0, 25°C Sulfolobus acidocaldarius
0.1287
-
4-nitrophenyl butanoate pH 8.0, 25°C Sulfolobus acidocaldarius
0.1517
-
4-nitrophenyl pentanoate pH 8.0, 25°C Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
4 * 32000, SDS-PAGE Sulfolobus acidocaldarius
117000
-
meniscus depletion sedimentation equilibrium in the analytical ultracentrifuge Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius Q7M529
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
158
-
-
Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O 8.8% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + acetate
-
?
4-nitrophenyl butanoate + H2O 83% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + butanoate
-
?
4-nitrophenyl decanoate + H2O 1.8% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + decanoate
-
?
4-nitrophenyl dodecanoate + H2O 0.8% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + dodecanoate
-
?
4-nitrophenyl hexanoate + H2O 75.4% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + hexanoate
-
?
4-nitrophenyl octanoate + H2O 11.5% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + octanoate
-
?
4-nitrophenyl pentanoate + H2O
-
Sulfolobus acidocaldarius 4-nitrophenol + pentanoate
-
?
4-nitrophenyl propionate + H2O 18% of the activity with 4-nitrophenyl pentanoate Sulfolobus acidocaldarius 4-nitrophenol + propionate
-
?
ethyl valerate + H2O
-
Sulfolobus acidocaldarius ethanol + valeric acid
-
?
hexyl valerate + H2O
-
Sulfolobus acidocaldarius hexanol + valeric acid
-
?
additional information no hydrolysis of triolein and ethyl acetate Sulfolobus acidocaldarius ?
-
?
pentyl acetate + H2O
-
Sulfolobus acidocaldarius pentanol + acetate
-
?
pentyl valerate + H2O not hydrolysed in the absence of Triton X-100 Sulfolobus acidocaldarius pentanol + valeric acid
-
?
triacetin + H2O
-
Sulfolobus acidocaldarius ?
-
?
tributyrin + H2O
-
Sulfolobus acidocaldarius ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 32000, SDS-PAGE Sulfolobus acidocaldarius

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
1 h, stable Sulfolobus acidocaldarius
90
-
1 h, 8% loss of activity Sulfolobus acidocaldarius
100
-
1 h, 50% loss of activity Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
Sulfolobus acidocaldarius

pI Value

Organism Comment pI Value Maximum pI Value
Sulfolobus acidocaldarius isoelectric focusing
-
4.9