Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour diffusion method | Sulfolobus acidocaldarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
eserine | 10 mM, 19% inhibition | Sulfolobus acidocaldarius | |
paraoxon | 0.01 mM, complete inhibition | Sulfolobus acidocaldarius | |
phenylmethanesulfonyl fluoride | 0.1 mM, complete inhibition | Sulfolobus acidocaldarius |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01036 | - |
4-nitrophenyl hexanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
0.0215 | - |
4-nitrophenyl acetate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
0.0338 | - |
4-nitrophenyl propionate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
0.0606 | - |
4-nitrophenyl octanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
0.1287 | - |
4-nitrophenyl butanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius | |
0.1517 | - |
4-nitrophenyl pentanoate | pH 8.0, 25°C | Sulfolobus acidocaldarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
4 * 32000, SDS-PAGE | Sulfolobus acidocaldarius |
117000 | - |
meniscus depletion sedimentation equilibrium in the analytical ultracentrifuge | Sulfolobus acidocaldarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sulfolobus acidocaldarius | Q7M529 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Sulfolobus acidocaldarius |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
- |
Sulfolobus acidocaldarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl acetate + H2O | 8.8% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + acetate | - |
? | |
4-nitrophenyl butanoate + H2O | 83% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + butanoate | - |
? | |
4-nitrophenyl decanoate + H2O | 1.8% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + decanoate | - |
? | |
4-nitrophenyl dodecanoate + H2O | 0.8% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + dodecanoate | - |
? | |
4-nitrophenyl hexanoate + H2O | 75.4% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + hexanoate | - |
? | |
4-nitrophenyl octanoate + H2O | 11.5% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + octanoate | - |
? | |
4-nitrophenyl pentanoate + H2O | - |
Sulfolobus acidocaldarius | 4-nitrophenol + pentanoate | - |
? | |
4-nitrophenyl propionate + H2O | 18% of the activity with 4-nitrophenyl pentanoate | Sulfolobus acidocaldarius | 4-nitrophenol + propionate | - |
? | |
ethyl valerate + H2O | - |
Sulfolobus acidocaldarius | ethanol + valeric acid | - |
? | |
hexyl valerate + H2O | - |
Sulfolobus acidocaldarius | hexanol + valeric acid | - |
? | |
additional information | no hydrolysis of triolein and ethyl acetate | Sulfolobus acidocaldarius | ? | - |
? | |
pentyl acetate + H2O | - |
Sulfolobus acidocaldarius | pentanol + acetate | - |
? | |
pentyl valerate + H2O | not hydrolysed in the absence of Triton X-100 | Sulfolobus acidocaldarius | pentanol + valeric acid | - |
? | |
triacetin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? | |
tributyrin + H2O | - |
Sulfolobus acidocaldarius | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 32000, SDS-PAGE | Sulfolobus acidocaldarius |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
1 h, stable | Sulfolobus acidocaldarius |
90 | - |
1 h, 8% loss of activity | Sulfolobus acidocaldarius |
100 | - |
1 h, 50% loss of activity | Sulfolobus acidocaldarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8.5 | - |
Sulfolobus acidocaldarius |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Sulfolobus acidocaldarius | isoelectric focusing | - |
4.9 |