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Literature summary for 3.1.1.1 extracted from
- Comparison and functional characterization of three homologous intracellular carboxylesterases of Bacillus subtilis (2005), J. Mol. Catal. B, 32, 261-270.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Bacillus subtilis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Bacillus subtilis |
| 34000 | - |
x * 34000, SDS-PAGE of both isoforms CesA and CesB | Bacillus subtilis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isoform CesA, CesB | - |
| Bacillus subtilis | - |
isoform NP | - |
| Bacillus subtilis 168 | - |
isoform CesA, CesB | - |
| Bacillus subtilis Thai I-8 | - |
isoform NP | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.01 | - |
isoform CesB, substrate beta-naphthylacetate, pH 7.5, 32°C | Bacillus subtilis |
| 0.03 | - |
isoform Cesb, substrate (R)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 0.04 | - |
isoform CesA, substrate (R)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 0.04 | - |
substrate (R)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 0.08 | - |
isoform CesA, substrate beta-naphthylacetate, pH 7.5, 32°C | Bacillus subtilis |
| 0.1 | - |
substrate beta-naphthylacetate, pH 7.5, 32°C | Bacillus subtilis |
| 0.17 | - |
isoform CesB, substrate (S)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 0.55 | - |
isoform CesB, substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 4.23 | - |
isoform CesA, substrate (S)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 4.6 | - |
substrate (S)-naproxen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 20.9 | - |
substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
| 25.4 | - |
isoform CesA, substrate Rac-ibuprofen methyl ester, pH 7.5, 32°C | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (+)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis | (+)-1,2-O-isopropylidenglycerol + caprylate | - |
? |  |
| (+)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis 168 | (+)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
| (+)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis Thai I-8 | (+)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
| (+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | - |
Bacillus subtilis | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
| (+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | no substrate for isoform CesB | Bacillus subtilis | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
| (+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | - |
Bacillus subtilis 168 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
| (+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | no substrate for isoform CesB | Bacillus subtilis 168 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
| (+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | - |
Bacillus subtilis Thai I-8 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
| (+/-)-1,2-O-isopropylidenglycerol butanoate + H2O | no substrate for isoform CesB | Bacillus subtilis Thai I-8 | (+/-)-1,2-O-isopropylidenglycerol + butanoate | - |
? | |
| (-)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
| (-)-1,2-O-isopropylidenglycerol caprylate + H2O | no substrate for isoform CesB | Bacillus subtilis | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
| (-)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis 168 | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
| (-)-1,2-O-isopropylidenglycerol caprylate + H2O | - |
Bacillus subtilis Thai I-8 | (-)-1,2-O-isopropylidenglycerol + caprylate | - |
? | |
| (R)-naproxen methyl ester + H2O | - |
Bacillus subtilis | (R)-naproxen + methanol | - |
? | |
| (S)-naproxen methyl ester + H2O | - |
Bacillus subtilis | (S)-naproxen + methanol | - |
? | |
| 2-naphthyl acetate + H2O | - |
Bacillus subtilis | 2-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Bacillus subtilis 168 | 2-naphthol + acetate | - |
? | |
| 2-naphthyl acetate + H2O | - |
Bacillus subtilis Thai I-8 | 2-naphthol + acetate | - |
? | |
| Rac-ibuprofen methyl ester + H2O | - |
Bacillus subtilis | Rac-ibuprofen + methanol | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Bacillus subtilis |
| ? | x * 34000, SDS-PAGE of both isoforms CesA and CesB | Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Bacillus subtilis |
| 30 | - |
isoform CesA | Bacillus subtilis |
| 40 | - |
isoform CesB | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
no enzymic activity above | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Bacillus subtilis |
| 7.5 | - |
both CesA and CesB | Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
inactive below | Bacillus subtilis |
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