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Literature summary for 3.1.1.1 extracted from

  • Mandrich, L.; Merone, L.; Pezzullo, M.; Cipolla, L.; Nicotra, F.; Rossi, M.; Manco, G.
    Role of the N terminus in enzyme activity, stability and specificity in thermophilic esterases belonging to the HSL family (2004), J. Mol. Biol., 345, 501-512.
    View publication on PubMed
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Saccharolobus solfataricus
the gene that has a deletion of about 60 residues at the N terminus(SSODELTAN) is expressed in Escherichia coli Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
M211S/R215L about 50% decrease both in kcat- and in KM-value Alicyclobacillus acidocaldarius
additional information truncated version of wild-type and mutant M211S/R215L lacking N-terminal 36 amino acids. Both truncated enzymes show reduction of temperature optimum to 50°C but pH-optimum of 7.1 similar to wild-type Alicyclobacillus acidocaldarius

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
 4-nitrophenyl hexanoate pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus
50
-
 p-nitrophenyl hexanoate pH 7.1, 70°C Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Alicyclobacillus acidocaldarius
-
 isoform EST2
-
Saccharolobus solfataricus
-
 isoform EST2
-
Saccharolobus solfataricus Q97VU2
-
-
Saccharolobus solfataricus P2 Q97VU2
-
-

Purification (Commentary)

Purification (Comment) Organism
mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus(SSODELTAN) Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.3
-
 pH 7.1, 70°C Saccharolobus solfataricus
4.3
-
 pH 7.1, 70°C, substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl dodecanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl dodecanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus P2 4-nitrophenol + dodecanoate
-
 ?
4-nitrophenyl hexanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus 4-nitrophenol + hexanoate
-
 ?
4-nitrophenyl hexanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus P2 4-nitrophenol + hexanoate
-
 ?
glyceryl tributyrate + H2O
-
 Saccharolobus solfataricus ?
-
 ?
glyceryl tributyrate + H2O
-
 Alicyclobacillus acidocaldarius ?
-
 ?
glyceryl tributyrate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus ?
-
 ?
glyceryl tributyrate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus P2 ?
-
 ?
glyceryl tridecanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus ?
-
 ?
glyceryl tridecanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus P2 ?
-
 ?
glyceryl trihexanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus ?
-
 ?
glyceryl trioctanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus ?
-
 ?
glyceryl trioleate + H2O
-
 Saccharolobus solfataricus ?
-
 ?
glyceryl trioleate + H2O
-
 Alicyclobacillus acidocaldarius ?
-
 ?
glyceryl trioleate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus ?
-
 ?
glyceryl trioleate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus P2 ?
-
 ?
p-nitrophenyl hexanoate + H2O
-
 Saccharolobus solfataricus p-nitrophenol + hexanoate
-
 ?
p-nitrophenyl hexanoate + H2O
-
 Alicyclobacillus acidocaldarius p-nitrophenol + hexanoate
-
 ?
S-methyl thiobutanoate + H2O activity with mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus methanol + thiobutanoate
-
 ?

Synonyms

Synonyms Comment Organism
EST2
-
 Saccharolobus solfataricus
esterase 2
-
 Saccharolobus solfataricus
SSO2517 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 truncated wild-type and M211S/R215L mutant enzyme lacking N-terminal 36 amino acids Alicyclobacillus acidocaldarius
70
-
-
 Saccharolobus solfataricus
70
-
 wild-type Alicyclobacillus acidocaldarius
70
-
 substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
60 75 60°C: 55% of maximal activity, 75°C: about 35% of maximal activity, substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 stable for more than 10 h Saccharolobus solfataricus
70
-
 wild-type, 3 h, no loss of activity, wild-type lacking N-terminal 36 amino acids, 70% residual activity, mutant M211S/R215L lacking N-terminal 36 amino acids, 30% residual activity Alicyclobacillus acidocaldarius
70
-
 stable for more than 10 h, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.47
-
 p-nitrophenyl hexanoate pH 7.1, 70°C Saccharolobus solfataricus
2.47
-
 4-nitrophenyl hexanoate pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus
5 15 p-nitrophenyl hexanoate pH 7.1, 50°C, wild-type lacking N-terminal 36 amino acids Alicyclobacillus acidocaldarius
508
-
 p-nitrophenyl hexanoate pH 7.1, 50°C, mutant M211S/R215L lacking N-terminal 36 amino acids Alicyclobacillus acidocaldarius
2300
-
 p-nitrophenyl hexanoate pH 7.1, 50°C, mutant M211S/R215L Alicyclobacillus acidocaldarius
4320
-
 p-nitrophenyl hexanoate pH 7.1, 50°C, wild-type Alicyclobacillus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.1
-
 at 70°C Saccharolobus solfataricus
7.1
-
 wild-type and N-terminal deletion mutant Alicyclobacillus acidocaldarius
7.1
-
 substrate: 4-nitrophenyl hexanoate, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus

pH Range

pH Minimum pH Maximum Comment Organism
4 10 pH 4.0: about 40% of maximal activity, pH 10.0: aboutv 40% of maximal activity, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
49.4
-
 4-nitrophenyl hexanoate pH 7.1, 70°C, mutant enzyme expressed from the gene that has a deletion of about 60 residues at the N terminus Saccharolobus solfataricus