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Literature summary for 3.1.1.1 extracted from
- Molecular cloning and characterization of two thermostable carboxyl esterases from Geobacillus stearothermophilus (2004), Gene, 329, 187-195.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | 1 mM, Est55, 33% stimulation, Est30, 7% stimulation | Geobacillus stearothermophilus |  |
| dithiothreitol | 1 mM, Est55, 45% stimulation, Est30, 719% stimulation | Geobacillus stearothermophilus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0005 | - |
p-nitrophenyl caproate | Est55, pH 8.5, 60°C | Geobacillus stearothermophilus | |
| 0.0022 | - |
p-nitrophenyl caproate | Est30, pH 8.5, 60°C | Geobacillus stearothermophilus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28338 | - |
Est30, 2 * 28338, calculated | Geobacillus stearothermophilus |
| 46000 | - |
Est55, gel filtration | Geobacillus stearothermophilus |
| 54867 | - |
Est55, 1 * 54867, calculated | Geobacillus stearothermophilus |
| 55000 | - |
Est30, gel filtration | Geobacillus stearothermophilus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
isoforms Est30, Est55 | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl acetate + H2O | Est55, 30% of the activity with 4-nitrophenyl butanoate, Est30, 20% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + acetate | - |
? | |
| 4-nitrophenyl butanoate + H2O | Est30, 65% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + butanoate | - |
? | |
| 4-nitrophenyl caprate + H2O | Est55, 1.6% of the activity with 4-nitrophenyl butanoate, Est30, 32% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + capric acid | - |
? | |
| 4-nitrophenyl caproate + H2O | Est55, 97% of the activity with 4-nitrophenyl butanoate, activation energy 35.7 kJ/mol. Est30, activation energy 101.9 kJ/mol | Geobacillus stearothermophilus | 4-nitrophenol + caproate | - |
? | |
| 4-nitrophenyl caprylate + H2O | Est55, 13% of the activity with 4-nitrophenyl butanoate, Est30, 50% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + caprylate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Geobacillus stearothermophilus | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | Est55, 0.6% of the activity with 4-nitrophenyl butanoate, Est30, 12% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + lauric acid | - |
? | |
| 4-nitrophenyl propanoate + H2O | Est55, 50% of the activity with 4-nitrophenyl butanoate, Est30, 45% of the activity with 4-nitrophenyl caproate | Geobacillus stearothermophilus | 4-nitrophenol + propanoate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | Est30, 2 * 28338, calculated | Geobacillus stearothermophilus |
| monomer | Est55, 1 * 54867, calculated | Geobacillus stearothermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Est30 | - |
Geobacillus stearothermophilus |
| Est55 | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
Est55 | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable for more than 2 h, Est55 and Est30 | Geobacillus stearothermophilus |
| 70 | - |
Est55, half-life 40 min, Est30, half-life 180 min | Geobacillus stearothermophilus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 38 | - |
p-nitrophenyl caproate | Est30, pH 8.5, 60°C | Geobacillus stearothermophilus | |
| 39760 | - |
p-nitrophenyl caproate | Est55, pH 8.5, 60°C | Geobacillus stearothermophilus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 9 | broad, Est55 | Geobacillus stearothermophilus |
| 9 | - |
Est30 | Geobacillus stearothermophilus |
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