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Literature summary for 3.1.1.1 extracted from

  • Sanishvili, R.; Yakunin, A.F.; Laskowski, R.A.; Skarina, T.; Evdokimova, E.; Doherty-Kirby, A.; Lajoie, G.A.; Thornton, J.M.; Arrowsmith, C.H.; Savchenko, A.; Joachimiak, A.; Edwards, A.M.
    Integrating structure, bioinformatics, and enzymology to discover function. BioH, a new carboxylesterase from Escherichia coli (2003), J. Biol. Chem., 278, 26039-26045.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.1.1

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli
expression of the selenomethionine enriched enzyme in Escherichia coli methionine auxotroph strain B834 (DE3) in supplemented M9 medium Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
-
 Escherichia coli
purified recombinant enzyme, by hanging drop vapour diffusion method, 0.002 ml of each, protein solution and precipitant solution, equilibration against reservoir solution containing 1.2 M sodium citrate trihydrate and 0.1 M Tris-HCl, pH 8.0, 21°C, 2-5 days, X-ray diffraction structure determination at 1.7 A resolution, structure analysis Escherichia coli

General Stability

General Stability Organism
stable Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Phenylmethylsulfonylfluoride
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.25
-
 4-nitrophenyl caproate pH 7.5, 37°C Escherichia coli
0.25
-
 p-nitrophenyl caproate +/- 0.02 Escherichia coli
0.29
-
 4-nitrophenyl acetate pH 7.5, 37°C Escherichia coli
0.29
-
 p-nitrophenyl acetate +/- 0.04 Escherichia coli
0.33
-
 4-nitrophenyl butyrate pH 7.5, 37°C Escherichia coli
0.33
-
 p-nitrophenyl butyrate +/- 0.06 Escherichia coli
0.35
-
 4-nitrophenyl propionate pH 7.5, 37°C Escherichia coli
0.35
-
 p-nitrophenyl propionate +/- 0.08 Escherichia coli
0.6
-
 4-nitrophenyl laurate pH 7.5, 37°C Escherichia coli
0.6
-
 p-nitrophenyl laurate +/- 0.13 Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
29152
-
 x * 29152, mass spectrometry Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Escherichia coli the enzyme BioH is involved in biotin biosynthesis ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 enzyme BioH, selenomethionine enriched protein, contains 2 Gly-Xaa-Ser-Xaa-Gly motifs
-
Escherichia coli P13001
-
-

Reaction

Reaction Comment Organism Reaction ID
a carboxylic ester + H2O = an alcohol + a carboxylate active site structure, catalytic triad consists of residues Ser82, His235, and Asp207 Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.19
-
 +/- 0.02, palmitoyl-CoA Escherichia coli
3.17
-
 +/- 0.34, p-nitrophenyl laurate Escherichia coli
8.4
-
 +/- 0.27, p-nitrophenyl caproate Escherichia coli
12.7
-
 +/- 1.5, p-nitrophenyl butyrate Escherichia coli
27.5
-
 +/- 2.5, p-nitrophenyl propionate Escherichia coli
38.9
-
 +/- 3.6, p-nitrophenyl acetate Escherichia coli

Storage Stability

Storage Stability Organism
4°C, 5% glycerol, 0.5 M NaCl, no loss in activity after several months Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl acetate + H2O best substrate Escherichia coli 4-nitrophenol + acetate
-
 ?
4-nitrophenyl butyrate + H2O
-
 Escherichia coli 4-nitrophenol + butyrate
-
 ?
4-nitrophenyl caproate + H2O
-
 Escherichia coli 4-nitrophenol + caproate
-
 ?
4-nitrophenyl caproate + H2O
-
 Escherichia coli DH5-alpha 4-nitrophenol + caproate
-
 ?
4-nitrophenyl laurate + H2O
-
 Escherichia coli 4-nitrophenol + laurate
-
 ?
4-nitrophenyl laurate + H2O
-
 Escherichia coli DH5-alpha 4-nitrophenol + laurate
-
 ?
4-nitrophenyl propionate + H2O
-
 Escherichia coli 4-nitrophenol + propionate
-
 ?
additional information enzyme shows low thioesterase activity, it cannot use free pimelic acid for pimeloyl-CoA synthesis, enzyme shows a broad substrate specificity with a preference for short chain substrates Escherichia coli ?
-
 ?
additional information the enzyme BioH is involved in biotin biosynthesis Escherichia coli ?
-
 ?
p-nitrophenyl acetate + H2O
-
 Escherichia coli p-nitrophenol + acetate
-
 ?
p-nitrophenyl acetate + H2O
-
 Escherichia coli DH5-alpha p-nitrophenol + acetate
-
 ?
p-nitrophenyl butyrate
-
 Escherichia coli p-nitrophenol + butyrate
-
 ?
p-nitrophenyl butyrate
-
 Escherichia coli DH5-alpha p-nitrophenol + butyrate
-
 ?
p-nitrophenyl caprate
-
 Escherichia coli p-nitrophenol + caprate
-
 ?
p-nitrophenyl caprate
-
 Escherichia coli DH5-alpha p-nitrophenol + caprate
-
 ?
p-nitrophenyl caproate
-
 Escherichia coli p-nitrophenol + caproate
-
 ?
p-nitrophenyl laurate
-
 Escherichia coli p-nitrophenol + laurate
-
 ?
p-nitrophenyl palmitate + H2O
-
 Escherichia coli p-nitrophenol + palmitate
-
 ?
p-nitrophenyl propionate
-
 Escherichia coli p-nitrophenol + propionate
-
 ?
p-nitrophenyl stearate
-
 Escherichia coli p-nitrophenol + stearate
-
 ?
palmitoyl-CoA
-
 Escherichia coli CoA + palmitate
-
 ?

Subunits

Subunits Comment Organism
? x * 29152, mass spectrometry Escherichia coli
monomer gel filtration Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
 palmitoyl-CoA
-
 Escherichia coli
1.5
-
 4-nitrophenyl laurate pH 7.5, 37°C Escherichia coli
1.5
-
 p-nitrophenyl laurate +/- 0.2 Escherichia coli
4
-
 4-nitrophenyl caproate pH 7.5, 37°C Escherichia coli
4
-
 p-nitrophenyl caproate +/- 0.1 Escherichia coli
6.1
-
 4-nitrophenyl butyrate pH 7.5, 37°C Escherichia coli
6.1
-
 p-nitrophenyl butyrate +/- 0.7 Escherichia coli
13.1
-
 4-nitrophenyl propionate pH 7.5, 37°C Escherichia coli
13.1
-
 p-nitrophenyl propionate +/- 1.2 Escherichia coli
18.5
-
 4-nitrophenyl acetate pH 7.5, 37°C Escherichia coli
18.5
-
 p-nitrophenyl acetate +/- 1.7 Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 8.5
-
 Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
6 10
-
 Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.15
-
 Phenylmethylsulfonylfluoride
-
 Escherichia coli