Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.1.1 extracted from

  • Kim, H.E.; Park, K.R.
    Purification and characterization of an esterase from Acinetobacter lwoffii I6C-1 (2002), Curr. Microbiol., 44, 401-405.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
Acinetobacter lwoffii
Co2+
-
Acinetobacter lwoffii
Cu2+
-
Acinetobacter lwoffii
diisopropyl fluorophosphate
-
Acinetobacter lwoffii
EDTA
-
Acinetobacter lwoffii
Fe2+
-
Acinetobacter lwoffii
Hg2+
-
Acinetobacter lwoffii
Mg2+
-
Acinetobacter lwoffii
Mn2+
-
Acinetobacter lwoffii
PMSF
-
Acinetobacter lwoffii
Zn2+
-
Acinetobacter lwoffii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
4-nitrophenyl butyrate pH 8.0, 37°C Acinetobacter lwoffii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Acinetobacter lwoffii 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
approximately, gel filtration Acinetobacter lwoffii

Organism

Organism UniProt Comment Textmining
Acinetobacter lwoffii
-
enzyme EstA
-
Acinetobacter lwoffii I6C-1
-
enzyme EstA
-

Purification (Commentary)

Purification (Comment) Organism
24.2fold Acinetobacter lwoffii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1440
-
purified enzyme Acinetobacter lwoffii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl butyrate + H2O
-
Acinetobacter lwoffii 4-nitrophenol + butyrate
-
?
4-nitrophenyl butyrate + H2O
-
Acinetobacter lwoffii I6C-1 4-nitrophenol + butyrate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Acinetobacter lwoffii

Synonyms

Synonyms Comment Organism
EstA
-
Acinetobacter lwoffii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Acinetobacter lwoffii

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 48 about 50% of maximal activity at 20°C and 48°C Acinetobacter lwoffii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
at least 1 h, stable Acinetobacter lwoffii
60
-
50 min, 50% remaining activity Acinetobacter lwoffii
70
-
40 min, 50% remaining activity Acinetobacter lwoffii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
Acinetobacter lwoffii

pH Range

pH Minimum pH Maximum Comment Organism
7 9.5 50% of maximal activity at pH 7.0 and pH 9.5 Acinetobacter lwoffii