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Literature summary for 3.1.1.1 extracted from
- Purification and characterization of a thermostable esterase from the moderate thermophile Bacillus circulans (2000), Appl. Microbiol. Biotechnol., 54, 173-179.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | usage of the enzyme as biocatalyst for biotransformations in non-aqueous media in industrial applications at high and also at moderate temperatures | Niallia circulans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| diethyldicarbonate | weak inhibition | Niallia circulans |  |
| EDTA | slight inhibition | Niallia circulans | |
| eserine | complete inhibition | Niallia circulans | |
| HgCl2 | complete inhibition | Niallia circulans | |
| p-hydroxymercuribenzoic acid | complete inhibition | Niallia circulans | |
| phenylmethylsulfonyl fluoride | complete inhibition | Niallia circulans | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
4-nitrophenyl octanoate | pH 8.0, 50°C | Niallia circulans | |
| 1.5 | - |
4-nitrophenyl propionate | pH 8.0, 50°C | Niallia circulans | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | contains no metal | Niallia circulans |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 30000 | - |
3 * 30000, SDS-PAGE | Niallia circulans |
| 95000 | - |
gel filtration | Niallia circulans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Niallia circulans | - |
moderate thermophile, strain MAS2 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 711fold, to homogeneity | Niallia circulans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 88.2 | - |
purified enzyme | Niallia circulans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl butyrate + H2O | - |
Niallia circulans | 4-nitrophenol + butyrate | - |
? | |
| 4-nitrophenyl hexanoate + H2O | - |
Niallia circulans | 4-nitrophenol + hexanoate | - |
? | |
| 4-nitrophenyl laurate + H2O | - |
Niallia circulans | 4-nitrophenol + lauric acid | - |
? | |
| 4-nitrophenyl myristate + H2O | - |
Niallia circulans | 4-nitrophenol + myristic acid | - |
? | |
| 4-nitrophenyl octanoate + H2O | - |
Niallia circulans | 4-nitrophenol + octanoate | - |
? | |
| 4-nitrophenyl palmitate + H2O | - |
Niallia circulans | 4-nitrophenol + palmitic acid | - |
? | |
| 4-nitrophenyl propionate + H2O | - |
Niallia circulans | 4-nitrophenol + propionate | - |
? | |
| additional information | maked specificity for mid-chain-length fatty acids of 3-8 C-atoms | Niallia circulans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| trimer | 3 * 30000, SDS-PAGE | Niallia circulans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Niallia circulans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | 74 | 50% of maximal activity at 30-37°C, maximal activity at 55°C, 60% activity at 74°C | Niallia circulans |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
completely stable up to | Niallia circulans |
| 71 | - |
50% inactivation after 1 h | Niallia circulans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51 | - |
4-nitrophenyl octanoate | pH 8.0, 50°C | Niallia circulans | |
| 144 | - |
4-nitrophenyl propionate | pH 8.0, 50°C | Niallia circulans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | 9.7 | above | Niallia circulans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 11 | - |
Niallia circulans |
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