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Literature summary for 2.8.4.1 extracted from

  • Soo, V.W.; McAnulty, M.J.; Tripathi, A.; Zhu, F.; Zhang, L.; Hatzakis, E.; Smith, P.B.; Agrawal, S.; Nazem-Bokaee, H.; Gopalakrishnan, S.; Salis, H.M.; Ferry, J.G.; Maranas, C.D.; Patterson, A.D.; Wood, T.K.
    Reversing methanogenesis to capture methane for liquid biofuel precursors (2016), Microb. Cell Fact., 15, 11.
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
environmental protection expression of methyl-coenzyme M reductase from an unculturable organism in Methanosarcina acetivorans to effectively run methanogenesis in reverse. Methanosarcina acetivorans cells heterologously producing methyl-coenzyme M reductase consume up to 9% of methane (corresponding to 109 micromol of methane) after 6 weeks of anaerobic growth on methane and utilize 10 mM FeCl3 as an electron acceptor. When incubated on methane for 5 days, high-densities of cells consume 15% methane (corresponding to 143 micromol of methane), and produce 10.3 mM acetate (corresponding to 52 micromol of acetate) uncultured bacterium
synthesis expression of methyl-coenzyme M reductase from an unculturable organism in Methanosarcina acetivorans to effectively run methanogenesis in reverse. Methanosarcina acetivorans cells heterologously producing methyl-coenzyme M reductase consume up to 9% of methane (corresponding to 109 ± 12 micromol of methane) after 6 weeks of anaerobic growth on methane and utilize 10 mM FeCl3 as an electron acceptor. When incubated on methane for 5 days, high-densities of cells consume 15% methane (corresponding to 143 ± 16 micromol of methane), and produce 10.3 mM acetate (corresponding to 52 ± 4 µmol of acetate) uncultured bacterium

Cloned(Commentary)

Cloned (Comment) Organism
-
uncultured bacterium

Organism

Organism UniProt Comment Textmining
uncultured bacterium
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i.e. anaerobic methanotrophic archaeal population 1, ANME-1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoM-S-S-CoB + methane
-
uncultured bacterium methyl-CoM + CoB
-
?

Synonyms

Synonyms Comment Organism
methyl-coenzyme M reductase
-
uncultured bacterium