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Literature summary for 2.8.4.1 extracted from

  • Cedervall, P.E.; Dey, M.; Pearson, A.R.; Ragsdale, S.W.; Wilmot, C.M.
    Structural insight into methyl-coenzyme M reductase chemistry using coenzyme B analogues (2010), Biochemistry, 49, 7683-7693.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.8.4.1

Crystallization (Commentary)

Crystallization (Comment) Organism
enzyme alone and in complex with substrates, sitting drop vapor diffusion method, using 100 mM Na-HEPES, pH 7.3-8.0, 150 mM magnesium acetate, and 20-22% (w/v) PEG 400 Methanothermobacter marburgensis

Inhibitors

Inhibitors Comment Organism Structure
O-phosphono-N-(5-sulfanylpentanoyl)-L-threonine
-
 Methanothermobacter marburgensis
O-phosphono-N-(6-sulfanylhexanoyl)-L-threonine
-
 Methanothermobacter marburgensis

Metals/Ions

Metals/Ions Comment Organism Structure
Nickel the enzyme contains nickel Methanothermobacter marburgensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CH3-S-CoM + HS-CoB Methanothermobacter marburgensis HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate CoM-S-S-CoB + methane
-
 ?
CH3-S-CoM + HS-CoB Methanothermobacter marburgensis OCM82 HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate CoM-S-S-CoB + methane
-
 ?

Organism

Organism UniProt Comment Textmining
Methanothermobacter marburgensis P11558 and P11560 and P11562 subunits alpha, beta and gamma
-
Methanothermobacter marburgensis OCM82 P11558 and P11560 and P11562 subunits alpha, beta and gamma
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothermobacter marburgensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CH3-S-CoM + HS-CoB HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate Methanothermobacter marburgensis CoM-S-S-CoB + methane
-
 ?
CH3-S-CoM + HS-CoB HS-CoB is N-(7-mercaptoheptanoyl)-L-threonine 3-O-phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB + methane
-
 ?
CH3-S-CoM + SH-CoB5 i.e. N-5-mercaptopentanoylthreonine phosphate Methanothermobacter marburgensis CoM-S-S-CoB5 + methane
-
 ?
CH3-S-CoM + SH-CoB5 i.e. N-5-mercaptopentanoylthreonine phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB5 + methane
-
 ?
CH3-S-CoM + SH-CoB6 i.e. N-6-mercaptohexanoylthreonine phosphate, slow substrate Methanothermobacter marburgensis CoM-S-S-CoB6 + methane
-
 ?
CH3-S-CoM + SH-CoB6 i.e. N-6-mercaptohexanoylthreonine phosphate, slow substrate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB6 + methane
-
 ?
CH3-S-CoM + SH-CoB8 i.e. N-8-mercaptooctanoylthreonine phosphate Methanothermobacter marburgensis CoM-S-S-CoB8 + methane
-
 ?
CH3-S-CoM + SH-CoB8 i.e. N-8-mercaptooctanoylthreonine phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB8 + methane
-
 ?
CH3-S-CoM + SH-CoB9 i.e. N-9-mercaptononanoylthreonine phosphate Methanothermobacter marburgensis CoM-S-S-CoB9 + methane
-
 ?
CH3-S-CoM + SH-CoB9 i.e. N-9-mercaptononanoylthreonine phosphate Methanothermobacter marburgensis OCM82 CoM-S-S-CoB9 + methane
-
 ?

Synonyms

Synonyms Comment Organism
MCR
-
 Methanothermobacter marburgensis
methyl-coenzyme M reductase
-
 Methanothermobacter marburgensis

Cofactor

Cofactor Comment Organism Structure
F-430 the enzyme contains the highly reduced nickel-tetrapyrrole coenzyme F430 Methanothermobacter marburgensis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0001
-
 O-phosphono-N-(6-sulfanylhexanoyl)-L-threonine pH and temperature not specified in the publication Methanothermobacter marburgensis