Literature summary for 2.8.4.1 extracted from

Ellermann, J.; Rospert, S.; Thauer, R.K.; Bokranz, M.; Klein, A.; Voges, M.; Berkessel, A.
Methyl-coenzyme-M reductase from Methanobacterium thermoautotrophicum (strain Marburg). Purity, activity and novel inhibitors (1989), Eur. J. Biochem., 184, 63-68.

Search for more literature for 2.8.4.1

Activating Compound

Activating Compound	Comment	Organism	Structure
ADP	in absence of reducing agents	Methanothermobacter thermautotrophicus
arsenate	in absence of reducing agents	Methanothermobacter thermautotrophicus
ATP	-	Methanothermobacter thermautotrophicus
Cobalamin	-	Methanothermobacter thermautotrophicus
dithiothreitol	-	Methanothermobacter thermautotrophicus
EDTA	in absence of reducing agents	Methanothermobacter thermautotrophicus
additional information	activated enzyme immediately loses 97% of activity when assayed in the absence of Ti(III) citrate, dithiothreitol and cobalamin, that shows the requirement of these reductants for maximal activity	Methanothermobacter thermautotrophicus
phosphate	in absence of reducing agents	Methanothermobacter thermautotrophicus
sulfate	in absence of reducing agents	Methanothermobacter thermautotrophicus
Ti(III) citrate	-	Methanothermobacter thermautotrophicus

Cloned(Commentary)

Cloned (Comment)	Organism
expression of two ORF gene products in Escherichia coli DS 410, expressed proteins are suggested to be important for enzyme activity, but neither stimulatory nor inhibitory effects of these gene products are determined	Methanothermobacter thermautotrophicus

Inhibitors

Inhibitors	Comment	Organism	Structure
2-azidoethanesulfonate	competitive, reversible	Methanothermobacter thermautotrophicus
2-bromoethanesulfonate	-	Methanothermobacter thermautotrophicus
3-azidopropanesulfonate	-	Methanothermobacter thermautotrophicus
3-bromopropanesulfonate	competitive, reversible, 50% inhibition at 0.00007 mM	Methanothermobacter thermautotrophicus
3-hydroxypropanesulfonate	-	Methanothermobacter thermautotrophicus
4-bromobutanesulfonate	-	Methanothermobacter thermautotrophicus
chloromethanesulfonate	-	Methanothermobacter thermautotrophicus
ethanol	concentrations above 0.005 ml/0.2 ml assay	Methanothermobacter thermautotrophicus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.075	-	coenzyme B	-	Methanothermobacter thermautotrophicus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ni(2+)	nickel-porphinoid-containing protein	Methanothermobacter thermautotrophicus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
additional information	-	operon contains two additional polypeptides of 15752 and 21210 Da with unknown functions	Methanothermobacter thermautotrophicus
37000	-	2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE	Methanothermobacter thermautotrophicus
48000	-	2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE	Methanothermobacter thermautotrophicus
66000	-	2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE	Methanothermobacter thermautotrophicus
300000	-	-	Methanothermobacter thermautotrophicus

Organism

Organism	UniProt	Comment	Textmining
Methanothermobacter thermautotrophicus	-	-	-
Methanothermobacter thermautotrophicus Marburg / DSM 2133	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
membrane filtration, on Mono Q, Superose-6 and Phenyl-Superose column	Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.5	-	purified, addition of Ti(III) citrate, dithiothreitol and cobalamin	Methanothermobacter thermautotrophicus
3	5	cell extract	Methanothermobacter thermautotrophicus

Storage Stability

Storage Stability	Organism
-80°C, under N2 atmosphere, 50 mM PIPES, pH 7.2, 40% v/v glycerol, placed in H2 gas phase, stable for weeks	Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
methyl-coenzyme M + coenzyme B	-	Methanothermobacter thermautotrophicus	methane + CoM-S-S-CoB	-	?
methyl-coenzyme M + coenzyme B	-	Methanothermobacter thermautotrophicus Marburg / DSM 2133	methane + CoM-S-S-CoB	-	?

Subunits

Subunits	Comment	Organism
hexamer	alpha2,beta2,gamma2	Methanothermobacter thermautotrophicus
hexamer	2 * 66000, 2 * 48000, 2 * 37000, SDS-PAGE	Methanothermobacter thermautotrophicus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
62	-	assay	Methanothermobacter thermautotrophicus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Methanothermobacter thermautotrophicus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.00005	-	3-bromopropanesulfonate	-	Methanothermobacter thermautotrophicus
0.001	-	2-azidoethanesulfonate	-	Methanothermobacter thermautotrophicus
0.004	-	2-bromoethanesulfonate	-	Methanothermobacter thermautotrophicus
0.006	-	4-bromobutanesulfonate	-	Methanothermobacter thermautotrophicus
0.04	-	3-azidopropanesulfonate	-	Methanothermobacter thermautotrophicus
0.25	-	chloromethanesulfonate	-	Methanothermobacter thermautotrophicus
4	-	3-hydroxypropanesulfonate	-	Methanothermobacter thermautotrophicus

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Release 2023.1 (January 2023)