Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, crystal form M obtained with 2-methyl-2,4-pentanediol grown within two months, form P grows from polyethylene glycol 400 within two weeks at 4°C, both crystal forms have one molecule per assymetric unit | Methanothermobacter thermautotrophicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ni(2+) | - |
Methanothermobacter thermautotrophicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
300000 | - |
- |
Methanothermobacter thermautotrophicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus | final step in methane formation in all methanogenic archaea | methane + CoM-S-S-CoB | - |
? | |
methyl coenzyme M + coenzyme B | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | final step in methane formation in all methanogenic archaea | methane + CoM-S-S-CoB | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanothermobacter thermautotrophicus | - |
- |
- |
Methanothermobacter thermautotrophicus Marburg / DSM 2133 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
anaerobic conditions | Methanothermobacter thermautotrophicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl coenzyme M + coenzyme B | final step in methane formation in all methanogenic archaea | Methanothermobacter thermautotrophicus | methane + CoM-S-S-CoB | - |
? | |
methyl coenzyme M + coenzyme B | final step in methane formation in all methanogenic archaea | Methanothermobacter thermautotrophicus Marburg / DSM 2133 | methane + CoM-S-S-CoB | - |
? |
Subunits | Comment | Organism |
---|---|---|
hexamer | alpha2,beta2,gamma2 | Methanothermobacter thermautotrophicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
F-430 | 2 mol coenzyme F-430/mol enzyme | Methanothermobacter thermautotrophicus | |
F-430 | 2 mol nickel porphinoid/mol enzyme, prosthetic group | Methanothermobacter thermautotrophicus |