Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | stimulates the reactivation | Methanosarcina thermophila | |
reduced ferredoxin | reactivation after purification | Methanosarcina thermophila | |
Ti(III) citrate | reactivation after isolation | Methanosarcina thermophila |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
methyl coenzyme M | - |
Methanosarcina thermophila | |
0.059 | - |
coenzyme B | - |
Methanosarcina thermophila |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | no activity found in membrane fraction | Methanosarcina thermophila | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma | Methanosarcina thermophila |
42000 | - |
1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma | Methanosarcina thermophila |
69000 | - |
1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma | Methanosarcina thermophila |
132000 | - |
nondenaturing slab gel electrophoresis | Methanosarcina thermophila |
141000 | - |
gel filtration | Methanosarcina thermophila |
144000 | - |
- |
Methanosarcina thermophila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl coenzyme M + coenzyme B | Methanosarcina thermophila | - |
methane + CoM-S-S-CoB | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanosarcina thermophila | - |
TM-1, acetate grown | - |
Purification (Comment) | Organism |
---|---|
sucrose density gradient cetrifugation, Q-Sepharose, Phenyl-Superose 5/5 column, anaerobic | Methanosarcina thermophila |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.224 | - |
- |
Methanosarcina thermophila |
Storage Stability | Organism |
---|---|
4°C, 11 h, fully active | Methanosarcina thermophila |
liquid N2, 50 mM potassium phosphate buffer, pH 7.0, 1 M potassium acetate, 10 mM 2-mercaptoethanol, 10% w/v glycerol | Methanosarcina thermophila |
three freeze-thaw cycles, fully active | Methanosarcina thermophila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
methyl coenzyme M + coenzyme B | - |
Methanosarcina thermophila | methane + CoM-S-S-CoB | - |
? |
Subunits | Comment | Organism |
---|---|---|
trimer | 1 * 69000, 1 * 42000 and 1 * 33000, alpha, beta and gamma | Methanosarcina thermophila |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methanosarcina thermophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
11 h, fully active | Methanosarcina thermophila |
23 | - |
N2 atmosphere, 2 h, 28% loss of original activity | Methanosarcina thermophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Methanosarcina thermophila |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
F-430 | 1 mol coenzyme F-430/mol enzyme | Methanosarcina thermophila |