Application | Comment | Organism |
---|---|---|
drug development | based on its role in the metabolism of microbial human pathogens, PPDK is a potential drug target | [Clostridium] symbiosum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4'-aminobutyl-5,7-dihydroxyflavone | displays selectivity for inhibition of PPDK versus other enzymes that utilize ATP and NAD+ | [Clostridium] symbiosum | |
4'-aminohexyl-5,7-dihydroxyflavone | a potent competitive PPDK inhibitor with an IC50 value of 0.0016 mM | [Clostridium] symbiosum | |
4'-aminopropyl-5,7-dihydroxyflavone | derivative specifically targets the ATP binding site and inhibits catalysis of only the PPDK + ATP + phosphate -> PPDK-P + AMP diphosphate partial reaction during single turnover experiments | [Clostridium] symbiosum | |
additional information | screening of substances that bind to the PPDK ATP-grasp domain active site reveals that flavone analogues are potent inhibitors of the Clostridium symbiosum PPDK. In silico modeling studies suggest that placement of a 36 carbon-tethered ammonium substituent at the 3'- or 4'-positions of 5,7-dihydroxyflavones result in favorable electrostatic interactions with the PPDK Mg-ATP binding site. Synthesis of polymethylene-tethered amine derivatives of 5,7-dihydroxyflavones | [Clostridium] symbiosum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | [Clostridium] symbiosum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | [Clostridium] symbiosum | - |
AMP + phosphoenolpyruvate + diphosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Clostridium] symbiosum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + pyruvate + phosphate | - |
[Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
r | |
ATP + pyruvate + phosphate | the enzyme forms phosphoenolpyruvate via two partial reactions: pyruvate phosphate dikinase + ATP + phosphate -> [pyruvate phosphate dikinase]-phosphate + AMP + diphosphate and [pyruvate phosphate dikinase]-phosphate + pyruvate -> phosphoenolpyruvate + pyruvate phosphate dikinase | [Clostridium] symbiosum | AMP + phosphoenolpyruvate + diphosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
PPDK | - |
[Clostridium] symbiosum |
pyruvate phosphate dikinase | - |
[Clostridium] symbiosum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
[Clostridium] symbiosum | |
ATP | - |
[Clostridium] symbiosum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | inhibitor steady-state kinetic analysis | [Clostridium] symbiosum | |
0.0016 | - |
4'-aminohexyl-5,7-dihydroxyflavone | pH and temperature not specified in the publication | [Clostridium] symbiosum |