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Literature summary for 2.7.8.7 extracted from
- The substrate promiscuity of a phosphopantetheinyl transferase SchPPT for coenzyme A derivatives and acyl carrier proteins (2016), Arch. Microbiol., 198, 193-197.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | SchPPT is a promiscuous PPTase and may be used on polyketide production in heterologous bacterial host and labeling of acyl-carrier proteins, ACPs | Streptomyces chattanoogensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene acpS, recombinant expression of N-terminaly His6-tagged enzyme in Escherichia coli BL21(DE3) from plasmid pHJ0024 | Streptomyces chattanoogensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Streptomyces chattanoogensis |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Streptomyces chattanoogensis | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | Streptomyces chattanoogensis L10 | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces chattanoogensis | H9N839 | - |
- |
| Streptomyces chattanoogensis L10 | H9N839 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminaly His6-tagged enzyme from Escherichia coli BL21(DE3) by nickel affinity chromatography | Streptomyces chattanoogensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | Streptomyces chattanoogensis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | usage of ACP substrate kbB-ACP4, an ACP in FK506 biosynthetic PKS/NRPS hybrid from Streptomyces tsukubaensis | Streptomyces chattanoogensis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | PPTases transfer 4'-phosphopantetheine from coenzyme A (CoA) to highly conserved serine residues in PCPs/ACPs, converting PCPs/ACPs from inactive apo-forms into active holo-forms | Streptomyces chattanoogensis L10 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| CoA-[4'-phosphopantetheine] + apo-[acyl-carrier protein] | usage of ACP substrate kbB-ACP4, an ACP in FK506 biosynthetic PKS/NRPS hybrid from Streptomyces tsukubaensis | Streptomyces chattanoogensis L10 | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
? | |
| additional information | substrate promiscuity of the phosphopantetheinyl transferase SchPPT for coenzyme A derivatives and acyl carrier proteins, SchPPT has a broad substrate specificity for ACPs | Streptomyces chattanoogensis | ? | - |
? | |
| additional information | substrate promiscuity of the phosphopantetheinyl transferase SchPPT for coenzyme A derivatives and acyl carrier proteins, SchPPT has a broad substrate specificity for ACPs | Streptomyces chattanoogensis L10 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AcpS | - |
Streptomyces chattanoogensis |
| group II PPTase | - |
Streptomyces chattanoogensis |
| holo-ACP synthase | UniProt | Streptomyces chattanoogensis |
| holo-[acyl-carrier-protein] synthase | UniProt | Streptomyces chattanoogensis |
| phosphopantetheinyl transferase | - |
Streptomyces chattanoogensis |
| PPTase | - |
Streptomyces chattanoogensis |
| SchPPT | - |
Streptomyces chattanoogensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Streptomyces chattanoogensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Streptomyces chattanoogensis |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | SchPPT is a group II PPTase | Streptomyces chattanoogensis |
| physiological function | phosphopantetheinyl transferases play an essential role in the biosyntheses of fatty acids, polyketides, and nonribosomal peptides | Streptomyces chattanoogensis |
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