Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Bacillus subtilis |
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas aeruginosa |
expressed in Escherichia coli BL21(DE3) cells | Pseudomonas putida |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
6-nitroso-1,2-benzopyrone | - |
Bacillus subtilis | |
6-nitroso-1,2-benzopyrone | - |
Pseudomonas aeruginosa | |
6-nitroso-1,2-benzopyrone | - |
Pseudomonas putida |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00062 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
0.0033 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
0.0038 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
0.0065 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
0.0086 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
0.011 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Bacillus subtilis | |
Mg2+ | required for activity | Pseudomonas aeruginosa | |
Mg2+ | required for activity | Pseudomonas putida |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Bacillus subtilis ATCC 6633 | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Pseudomonas putida | - |
- |
- |
Pseudomonas putida KT 2240 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Bacillus subtilis |
Ni-NTA column chromatography | Pseudomonas aeruginosa |
Ni-NTA column chromatography | Pseudomonas putida |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Pseudomonas aeruginosa | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Pseudomonas putida | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Pseudomonas putida KT 2240 | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? | |
CoA-[4'-phosphopantetheine] + apo-[BpsA protein] | - |
Bacillus subtilis ATCC 6633 | adenosine 3',5'-bisphosphate + holo-[BpsA protein] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
4-phosphopantetheinyl transferase | - |
Bacillus subtilis |
4-phosphopantetheinyl transferase | - |
Pseudomonas aeruginosa |
4-phosphopantetheinyl transferase | - |
Pseudomonas putida |
PcpS | - |
Pseudomonas aeruginosa |
PP1183 | - |
Pseudomonas putida |
PPTase | - |
Bacillus subtilis |
PPTase | - |
Pseudomonas aeruginosa |
PPTase | - |
Pseudomonas putida |
Sfp | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
0.035 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
0.037 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
0.075 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
0.183 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
0.683 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
4'-phosphopantetheine | - |
Bacillus subtilis | |
4'-phosphopantetheine | - |
Pseudomonas aeruginosa | |
4'-phosphopantetheine | - |
Pseudomonas putida |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00042 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
0.00053 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
0.0024 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
0.003 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
0.003 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
0.0052 | - |
6-nitroso-1,2-benzopyrone | in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0021 | - |
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | 6-nitroso-1,2-benzopyrone | |
0.0037 | - |
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | 6-nitroso-1,2-benzopyrone | |
0.004 | - |
in the presence of 0.0025 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | 6-nitroso-1,2-benzopyrone | |
0.0091 | - |
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | 6-nitroso-1,2-benzopyrone | |
0.0098 | - |
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | 6-nitroso-1,2-benzopyrone | |
0.0108 | - |
in the presence of 0.01 mM CoA, in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | 6-nitroso-1,2-benzopyrone |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.3 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
3.5 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis | |
20 | - |
CoA-[4'-phosphopantetheine] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
21.7 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas putida | |
107 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Pseudomonas aeruginosa | |
108 | - |
apo-[BpsA protein] | in 100 mM Tris-HCl, pH 7.8, at 30°C | Bacillus subtilis |