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Literature summary for 2.7.8.7 extracted from

  • Zhou, Z.; Cironi, P.; Lin, A.J.; Xu, Y.; Hrvatin, S.; Golan, D.E.; Silver, P.A.; Walsh, C.T.; Yin, J.
    Genetically encoded short peptide tags for orthogonal protein labeling by Sfp and AcpS phosphopantetheinyl transferases (2007), ACS Chem. Biol., 2, 337-346.
    View publication on PubMed
Search for more literature for 2.7.8.7

Application

Application Comment Organism
biotechnology identification of short peptide tags of 12 residues as efficient substrate for site-specific protein labeling catalyzed by enzyme Bacillus subtilis
biotechnology identification of short peptide tags of 12 residues as efficient substrate for site-specific protein labeling catalyzed by enzyme Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0515
-
 GDSLSWLLRLLN
-
 Bacillus subtilis
0.0618
-
 GDSLSWLVRLLN
-
 Bacillus subtilis
0.076
-
 GDSLSWLLRLLN
-
 Escherichia coli
0.0772
-
 GDSLSWLVRCLN
-
 Escherichia coli
0.105
-
 GDSLSWLVRLLN
-
 Escherichia coli
0.108
-
 GDSLSWLLRCLN
-
 Escherichia coli
0.117
-
 GDSLDMLEWSLM
-
 Escherichia coli
0.12
-
 GDSLSWLLRCLN
-
 Bacillus subtilis
0.123
-
 DSLEFIASKLA
-
 Bacillus subtilis
0.139
-
 GDSLSWLVRCLN
-
 Bacillus subtilis
0.221
-
 GDSLSWLLRSLN
-
 Bacillus subtilis
0.242
-
 DSLEFIASKLA
-
 Escherichia coli
0.254
-
 GDSLSWLLRSLN
-
 Escherichia coli
0.534
-
 GDSLDMLEWSLM
-
 Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biotin-CoA + DSLEFIASKLA
-
 Bacillus subtilis D-(biotinyl-4'-phosphopantetheinyl)SLEFIASKLA + ?
-
 ?
biotin-CoA + DSLEFIASKLA
-
 Escherichia coli D-(biotinyl-4'-phosphopantetheinyl)SLEFIASKLA + ?
-
 ?
biotin-CoA + GDSLDMLEWSLM
-
 Bacillus subtilis GD-(biotinyl-4'-phosphopantetheinyl)SLDMLEWSLM + ?
-
 ?
biotin-CoA + GDSLDMLEWSLM
-
 Escherichia coli GD-(biotinyl-4'-phosphopantetheinyl)SLDMLEWSLM + ?
-
 ?
biotin-CoA + GDSLSWLLRCLN
-
 Bacillus subtilis GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRCLN + ?
-
 ?
biotin-CoA + GDSLSWLLRCLN
-
 Escherichia coli GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRCLN + ?
-
 ?
biotin-CoA + GDSLSWLLRLLN
-
 Bacillus subtilis GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRLLN + ?
-
 ?
biotin-CoA + GDSLSWLLRLLN
-
 Escherichia coli GD-(biotinyl-4'-phosphopantetheinyl)SLSWLLRLLN + ?
-
 ?
biotin-CoA + GDSLSWLLRSLN
-
 Bacillus subtilis GD-(biotinyl-4'-[N-{2-[2-(2-aminoethoxy)ethoxy]ethyl}-3-(2,5-dioxopyrrolidin-1-yl)propanamide]phosphopantetheinyl)SLSWLLRSLN + ?
-
 ?
biotin-CoA + GDSLSWLLRSLN
-
 Escherichia coli GD-(biotinyl-4'-[N-{2-[2-(2-aminoethoxy)ethoxy]ethyl}-3-(2,5-dioxopyrrolidin-1-yl)propanamide]phosphopantetheinyl)SLSWLLRSLN + ?
-
 ?
biotin-CoA + GDSLSWLVRCLN
-
 Bacillus subtilis GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRCLN + ?
-
 ?
biotin-CoA + GDSLSWLVRCLN
-
 Escherichia coli GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRCLN + ?
-
 ?
biotin-CoA + GDSLSWLVRLLN
-
 Bacillus subtilis GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRLLN + ?
-
 ?
biotin-CoA + GDSLSWLVRLLN
-
 Escherichia coli GD-(biotinyl-4'-phosphopantetheinyl)SLSWLVRLLN + ?
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0005
-
 GDSLSWLVRLLN
-
 Escherichia coli
0.0005
-
 GDSLSWLLRLLN
-
 Escherichia coli
0.0007
-
 GDSLSWLVRCLN
-
 Escherichia coli
0.001
-
 GDSLSWLLRCLN
-
 Escherichia coli
0.0013
-
 GDSLSWLLRSLN
-
 Escherichia coli
0.004
-
 GDSLDMLEWSLM
-
 Bacillus subtilis
0.013
-
 DSLEFIASKLA
-
 Escherichia coli
0.03
-
 GDSLDMLEWSLM
-
 Escherichia coli
0.05
-
 GDSLSWLVRLLN
-
 Bacillus subtilis
0.07
-
 GDSLSWLVRCLN
-
 Bacillus subtilis
0.14
-
 GDSLSWLLRSLN
-
 Bacillus subtilis
0.17
-
 GDSLSWLLRCLN
-
 Bacillus subtilis
0.17
-
 GDSLSWLLRLLN
-
 Bacillus subtilis
0.18
-
 DSLEFIASKLA
-
 Bacillus subtilis