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Literature summary for 2.7.8.7 extracted from

  • Mofid, M.R.; Finking, R.; Marahiel, M.A.
    Recognition of hybrid peptidyl carrier proteins/acyl carrier proteins in nonribosomal peptide synthetase modules by the 4'-phosphopantetheinyl transferases AcpS and Sfp (2002), J. Biol. Chem., 277, 17023-17031.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
sfp and acpS cloned and overproduced in Escherichia coli M15 Bacillus subtilis

Crystallization (Commentary)

Crystallization (Comment) Organism
co-crystallized with CoA and ACP Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0002
-
apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme AcpS, 0.002-0.008 mM substrate Bacillus subtilis
0.0014
-
apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme Sfp, 0.002-0.008 mM substrate Bacillus subtilis
0.00445
-
apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp Bacillus subtilis
0.0216
-
apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme AcpS, apo-hPCP Bacillus subtilis
0.026
-
apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp, apo-hPCP Bacillus subtilis
0.038
-
apo-ACP pH 8.8, 37°C, isoenzyme Sfp, 0.02-0.2 mM substrate Bacillus subtilis
0.068
-
apo-ACP pH 8.8, 37°C, isoenzyme AcpS, 0.02-0.2 mM substrate Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
CoA + apo-[acyl-carrier protein] Bacillus subtilis
-
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant isoenzymes AcpS and Sfp, expressed in Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
CoA + apo-[acyl-carrier protein]
-
Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein]
-
r
CoA + apo-[peptidyl carrier protein]
-
Bacillus subtilis adenosine 3',5'-bisphosphate + holo-[peptidyl-carrier protein]
-
r
additional information Sfp type exhibits an extraordinarily broad substrate specificity Bacillus subtilis ?
-
?

Subunits

Subunits Comment Organism
monomer isoenzyme AcpS Bacillus subtilis
trimer isoenzyme Sfp Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0283
-
apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme Sfp, 0.002-0.008 mM substrate Bacillus subtilis
0.208
-
apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme Sfp, 0.02-0.2 mM substrate Bacillus subtilis
0.239
-
apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme AcpS, apo-hPCP Bacillus subtilis
0.367
-
apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme AcpS, 0.002-0.008 mM substrate Bacillus subtilis
1.6
-
apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp Bacillus subtilis
1.6
-
apo-peptidyl carrier protein pH 8.8, 37°C, isoenzyme Sfp, apo-hPCP Bacillus subtilis
2.08
-
apo-acyl-carrier protein pH 8.8, 37°C, isoenzyme AcpS, 0.02-0.2 mM substrate Bacillus subtilis