Cloned (Comment) | Organism |
---|---|
cloned in Escherichia coli DH5alpha and overexpressed in Escherichia coli MV1190/pUC8-Sfp | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D107A | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
D107E | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
E151A | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
G105A | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
G105D | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
K155A | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
W147A | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
W147F | site-directed mutagenesis of the sfp gene, constructed using the SOE method | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no substantial inhibition of Sfp by apo-[PCPH6SrfB1.18] | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0018 | - |
apo-[PCPH6SrfB2.18] | pH 6.0, 37°C | Bacillus subtilis | |
0.005 | - |
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] | pH 6.0, 37°C | Bacillus subtilis | |
0.006 | - |
apo-acyl-carrier protein | pH 6.0, 37°C | Bacillus subtilis | |
0.007 | - |
CoA | pH 6.0, 37°C | Bacillus subtilis | |
0.016 | - |
apo-[EntB-ArCP-H6 Escherichia coli] | pH 6.0, 37°C | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | Bacillus subtilis | enzyme Sfp required for production of the lipoheptapeptide antibiotic surfactin | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, expressed in Escherichia coli MV1190/pUC8-Sfp | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetonyldethio-CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
acetyl-CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | CoA + acetyl-[acyl-carrier protein] | - |
r | |
benzoyl-CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | CoA + benzoyl-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | enzyme Sfp required for production of the lipoheptapeptide antibiotic surfactin | Bacillus subtilis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[EntB-ArCP-H6 E. coli] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[PCPH6SrfB1.18] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[PCPH6SrfB2.18] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
CoA-[4'-phosphopantetheine] + apo-[EntB-ArCP-H6 Escherichia coli] | - |
Bacillus subtilis | ? | - |
? | |
desulfo-CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
homocysteamine-CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r | |
additional information | 3'-dephospho-CoA is no substrate | Bacillus subtilis | ? | - |
? | |
phenylacetyl-CoA + apo-[acyl-carrier protein] | - |
Bacillus subtilis | ? + holo-[acyl-carrier protein] | - |
r |
Synonyms | Comment | Organism |
---|---|---|
phosphopantetheinyl transferase | - |
Bacillus subtilis |
PPTase | - |
Bacillus subtilis |
Sfp | - |
Bacillus subtilis |
surfactin synthetase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0967 | - |
apo-acyl-carrier protein | pH 6.0, 33°C | Bacillus subtilis | |
0.933 | - |
apo-[PCPH6SrfB2.18] | pH 6.0, 37°C | Bacillus subtilis | |
1.08 | - |
apo-[EntB-ArCP-H6 Escherichia coli] | pH 6.0, 37°C | Bacillus subtilis | |
1.17 | - |
apo-[Lys2-PCP-H6 Saccharomyces cerevisiae] | pH 6.0, 37°C | Bacillus subtilis | |
1.7 | - |
apo-acyl-carrier protein | pH 6.0, 37°C | Bacillus subtilis | |
1.73 | - |
apo-[PCPH6SrfB1.18] | pH 6.0, 37°C | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus subtilis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 8.5 | less than 20% of maximum activity at both pH 5.0 and 7.0, 50% of activity maximum at pH 5.5 and pH 6.8 | Bacillus subtilis |