General Stability | Organism |
---|---|
quite heat labile, unstable during chromatographic procedures intended for its purification, can only be partially overcome by Mg2+ and dithiothreitol, addition of ethylene glycol, glycerol, EDTA or proteinase inhibitors failed to stabilize the plant enzyme | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3',5'-ADP | inhibited in vitro by the reaction product | Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
apo-acyl-carrier protein | pH 8.0, 37°C | Spinacia oleracea | |
0.072 | - |
apo-acyl-carrier protein | pH 8.0, 37°C | Spinacia oleracea |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Spinacia oleracea | 5829 | - |
cytosol | - |
Ricinus communis | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requires Mg2+ or Mn2+ for activity, Km for Mg2+ 3.0 mM | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | Spinacia oleracea | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | Ricinus communis | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ricinus communis | - |
castor-oil-seed, L. cv. Baker 296 | - |
Spinacia oleracea | - |
spinach, L. cv. 424 | - |
Purification (Comment) | Organism |
---|---|
partially | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endosperm | - |
Ricinus communis | - |
leaf | - |
Spinacia oleracea | - |
seed | - |
Ricinus communis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | - |
Spinacia oleracea | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | - |
Ricinus communis | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
additional information | specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive | Spinacia oleracea | ? | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
- |
Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 8.8 | half maximal activity at pH 7.4 | Spinacia oleracea |