Application | Comment | Organism |
---|---|---|
analysis | enzyme can be utilized in an assay for apo-ACP in biological material | Escherichia coli |
General Stability | Organism |
---|---|
very unstable, markedly protected from inactivation by the presence of half-saturating concentrations of reduced CoA | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
apo-acyl-carrier protein | - |
Escherichia coli | |
0.15 | - |
CoA | pH 8.0, 33°C | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Escherichia coli | 5737 | - |
cytoplasm | - |
Saccharomyces cerevisiae | 5737 | - |
cytoplasm | - |
Clostridium kluyveri | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | maximal activity at 0.025 mM | Escherichia coli | |
additional information | CuSO4, CdCl2 and CrCl2 does not stimulate the reaction at any ceoncentration, trivalent metal cations such as FeCl3 or AlCl3 are ineffective | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | Escherichia coli | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | Saccharomyces cerevisiae | - |
adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Clostridium kluyveri | - |
- |
- |
Escherichia coli | - |
- |
- |
Rattus norvegicus | - |
rat | - |
Saccharomyces cerevisiae | - |
yeast | - |
Purification (Comment) | Organism |
---|---|
partially | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + apo-[acyl-carrier protein] | - |
Escherichia coli | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
CoA + apo-[acyl-carrier protein] | - |
Saccharomyces cerevisiae | adenosine 3',5'-bisphosphate + holo-[acyl-carrier protein] | - |
r | |
additional information | specificity of the holo-ACP synthetase is not examined in detail, only CoA is the donor of the 4'-phosphopantetheine moiety, dephospho-CoA is essentially inactive | Escherichia coli | ? | - |
? |