Cloned (Comment) | Organism |
---|---|
gene pgsA and pgsA2, amino acid sequence comparisons, quantitative RT-PCR expression analysis | Chlamydomonas reinhardtii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas reinhardtii | A8JEJ8 | - |
- |
Chlamydomonas reinhardtii CC-503 | A8JEJ8 | - |
- |
Synonyms | Comment | Organism |
---|---|---|
PGPS | - |
Chlamydomonas reinhardtii |
pgsA | - |
Chlamydomonas reinhardtii |
phosphatidylglycerophosphate synthase | - |
Chlamydomonas reinhardtii |
Organism | Comment | Expression |
---|---|---|
Chlamydomonas reinhardtii | partial functional complementation of Synechocystis sp. PCC6803 gene pgsA mutant individually by CrPGP1 and CrPGP2 (pgsA/CrPGP1 and pgsA/CrPGP2) rescueing the phosphatidylglycerophosphate-dependent growth phenotype, but the phosphatidylglycerophosphate level and its fatty acid composition are not fully rescued in the complemented strains. As well, oxygen evolution activity is not fully recovered, although electron transport activity of photosystem II is restored to the wild-type level, differential response of CrPGP1 and CrPGP2 expression to phosphorus and nitrogen deficiency, overview. Cloning in vector which is designed to incorporate a gene of interest at a neutral site (slr2031) with the psbA2 (slr1311) promoter and a chloramphenicol-resistance cassette | additional information |
General Information | Comment | Organism |
---|---|---|
malfunction | the Synechocystis sp.PCC 6803 pgsA mutant isdefective in PGPS activity | Chlamydomonas reinhardtii |
metabolism | phosphatidylglycerophosphate synthase, PGPS, is the rate-limiting enzyme in phosphatidylglycerophosphate biosynthesis in Chlamydomonas reinhardtii | Chlamydomonas reinhardtii |
physiological function | distinct and overlapping function of PGPS in cyanobacteria and eukaryotic algae. Heterologous complementation of Synechocystis sp. PCC6803 gene pgsA mutant individually by CrPGP1 and CrPGP2 rescues the phosphatidylglycerophosphate-dependent growth phenotype, but the phosphatidylglycerophosphate level and its fatty acid composition are not fully rescued in the complemented strains. As well, oxygen evolution activity is not fully recovered, although electron transport activity of photosystem II is restored to the wild-type level, differential response of CrPGP1 and CrPGP2 expression to phosphorus and nitrogen deficiency. LipidCompositionof pgsA/CrPGP1 and pgsA/CrPGP2, the rescued mutant content is reduced compared to the wild-type, overview | Chlamydomonas reinhardtii |