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Literature summary for 2.7.7.9 extracted from
- Molecular cloning, characterization, and comparison of UDP-glucose pyrophosphorylase from Gracilaria chouae and Saccharina japonica (2016), J. Appl. Phycol., 28, 2051-2059.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | about 40% inhibition | Saccharina japonica |  |
| Cu2+ | complete inhibition | Gracilaria chouae | |
| Cu2+ | complete inhibition | Saccharina japonica | |
| Mn2+ | about 40% inhibition | Saccharina japonica | |
| Pb2+ | complete inhibition | Gracilaria chouae | |
| Pb2+ | complete inhibition | Saccharina japonica | |
| Zn2+ | about 50% inhibition | Gracilaria chouae | |
| Zn2+ | about 40% inhibition | Saccharina japonica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00167 | - |
UDP-glucose | pH 7.5, temperature not specified in the publication | Gracilaria chouae | |
| 0.00433 | - |
UDP-glucose | pH 7.5, temperature not specified in the publication | Saccharina japonica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight stimulation | Gracilaria chouae | |
| Mg2+ | about 3fold stimulation | Gracilaria chouae | |
| Mg2+ | about 4fold stimulation | Saccharina japonica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72000 | - |
x * 72000, SDS-PAGE | Gracilaria chouae |
| 72000 | - |
x * 72000, SDS-PAGE | Saccharina japonica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gracilaria chouae | A0A0S3X5N5 | - |
- |
| Saccharina japonica | A0A0S3WYP3 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + UDP-glucose | - |
Gracilaria chouae | UTP + alpha-D-glucose 1-phosphate | - |
? | |
| diphosphate + UDP-glucose | - |
Saccharina japonica | UTP + alpha-D-glucose 1-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 72000, SDS-PAGE | Gracilaria chouae |
| ? | x * 72000, SDS-PAGE | Saccharina japonica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Saccharina japonica |
| 40 | - |
- |
Gracilaria chouae |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.98 | - |
UDP-glucose | pH 7.5, temperature not specified in the publication | Gracilaria chouae | |
| 19.98 | - |
UDP-glucose | pH 7.5, temperature not specified in the publication | Saccharina japonica | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Gracilaria chouae |
| 8 | - |
- |
Saccharina japonica |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4610 | - |
UDP-glucose | pH 7.5, temperature not specified in the publication | Saccharina japonica | |
| 11980 | - |
UDP-glucose | pH 7.5, temperature not specified in the publication | Gracilaria chouae | |
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