Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, recombinant overepression in Streptococcus zooepidemicus, expression of His-tagged enzyme in Escherichia coli | Streptococcus equi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0085 | - |
UTP | pH 7.5, 37°C | Streptococcus equi | |
0.0364 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C | Streptococcus equi |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | Streptococcus equi | - |
diphosphate + UDP-glucose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus equi | Q8GQP8 | subsp. zooepidemicus. i.e. Streptococcus zooepidemicus | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme in Escherichia coli by nickel affinity chromatography | Streptococcus equi |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UTP + alpha-D-glucose 1-phosphate | - |
Streptococcus equi | diphosphate + UDP-glucose | - |
r |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose pyrophosphorylase | - |
Streptococcus equi |
UGPase | - |
Streptococcus equi |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Streptococcus equi |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
48.81 | - |
alpha-D-glucose 1-phosphate | pH 7.5, 37°C | Streptococcus equi | |
69.05 | - |
UTP | pH 7.5, 37°C | Streptococcus equi |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Streptococcus equi |
General Information | Comment | Organism |
---|---|---|
additional information | structure-function analysis, homology modeling | Streptococcus equi |
physiological function | the enzyme plays an important role in Streptococcus zooepidemicus cell envelope hyaluronic acid biosynthesis and it is also recognized as a virulence determinant in several bacterial species | Streptococcus equi |