Literature summary for 2.7.7.9 extracted from

Daran, J.M.; Dallies, N.; Thines-Sempoux, D.; Paquet, V.; Francois, J.
Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiae (1995), Eur. J. Biochem., 233, 520-530.

Cloned(Commentary)

Cloned (Comment)	Organism
DNA sequence determination and analysis, gene disruption and depletion of activity is performed, overexpression from multicopy plasmid in haploid strains, on glucose 40fold increased enzyme activity leads to 2fold increase in the concentration of glycogen and UDP-D-glucose, on galactose a 40fold increased activity leads to several effects, e.g. 3fold reduction of growth rate, 3-5fold increase in UDP-D-glucose, UDP-D-galactose and alpha-D-galactose 1-phosphate concentrations, a higher sensitivity to calcofluor white and an increase in the degree of protein glycosylation	Saccharomyces cerevisiae

Cloned (Comment)

Organism

DNA sequence determination and analysis, gene disruption and depletion of activity is performed, overexpression from multicopy plasmid in haploid strains, on glucose 40fold increased enzyme activity leads to 2fold increase in the concentration of glycogen and UDP-D-glucose, on galactose a 40fold increased activity leads to several effects, e.g. 3fold reduction of growth rate, 3-5fold increase in UDP-D-glucose, UDP-D-galactose and alpha-D-galactose 1-phosphate concentrations, a higher sensitivity to calcofluor white and an increase in the degree of protein glycosylation

Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
UTP + alpha-D-glucose 1-phosphate	Saccharomyces cerevisiae	enzyme is essential for cell viability	diphosphate + UDP-glucose	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	-	gene UGP1, i.e. open reading frame YKL248	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	activity of several mutant strains in vivo	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
UTP + alpha-D-galactose 1-phosphate	-	Saccharomyces cerevisiae	diphosphate + UDP-galactose	-	r
UTP + alpha-D-glucose 1-phosphate	-	Saccharomyces cerevisiae	diphosphate + UDP-glucose	-	r
UTP + alpha-D-glucose 1-phosphate	enzyme is essential for cell viability	Saccharomyces cerevisiae	diphosphate + UDP-glucose	-	?

Synonyms

Synonyms	Comment	Organism
UGPase	-	Saccharomyces cerevisiae