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Literature summary for 2.7.7.9 extracted from

  • Gustafson, G.L.; Gander, J.E.
    Uridine diphosphate glucose pyrophosphorylase from Sorghum vulgare. Purification and kinetic properties (1972), J. Biol. Chem., 247, 1387-1397.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol
-
Sorghum bicolor
additional information no activation by 3-phosphoglycerate Sorghum bicolor
additional information no activation by fructose 1,6-bisphosphate, fructose 6-phosphate, phosphoenolpyruvate or p-hydroxymandelonitrile-beta-D-glucopyranoside Sorghum bicolor

Inhibitors

Inhibitors Comment Organism Structure
alpha-D-glucose 1-phosphate product inhibition Sorghum bicolor
Co2+ in excess of diphosphate-concentration, activates at lower concentrations Sorghum bicolor
Mg-diphosphate product inhibition Sorghum bicolor
Mn2+ in excess of diphosphate-concentration, activates at lower concentrations Sorghum bicolor
UDP-glucose product inhibition Sorghum bicolor
UTP product inhibition Sorghum bicolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Sorghum bicolor
0.03
-
UTP pH 8.5, 37°C Sorghum bicolor
0.03 0.048 alpha-D-glucose 1-phosphate pH 8.5, 37°C Sorghum bicolor
0.05 0.066 UDP-glucose
-
Sorghum bicolor
0.054
-
diphosphate pH 8.5, 37°C Sorghum bicolor

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation Sorghum bicolor
Co2+ Co2+ in excess of diphosphate-concentration inhibits Sorghum bicolor
Mg2+ requirement Sorghum bicolor
Mg2+ increasing MgSO4 concentrations shift the apparent equilibrium toward UDP-glucose synthesis Sorghum bicolor
Mg2+ Mg-diphosphate is the actual substrate Sorghum bicolor
Mn2+ activation Sorghum bicolor
Mn2+ UDP-glucose synthesis: Mn2+ in excess of diphosphate-concentration inhibits Sorghum bicolor
Mn2+ about 25% as effective as Mg2+ Sorghum bicolor
additional information requirement for divalent cations Sorghum bicolor

Organism

Organism UniProt Comment Textmining
Sorghum bicolor
-
cv. Rox Orange
-

Purification (Commentary)

Purification (Comment) Organism
-
Sorghum bicolor

Reaction

Reaction Comment Organism Reaction ID
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose sequential ordered bi bi reaction mechanism Sorghum bicolor

Source Tissue

Source Tissue Comment Organism Textmining
seedling etiolated Sorghum bicolor
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1200
-
purified enzyme Sorghum bicolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + CDP-glucose no activity Sorghum bicolor CTP + alpha-D-glucose 1-phosphate
-
?
diphosphate + GDP-glucose no activity Sorghum bicolor GTP + alpha-D-glucose 1-phosphate
-
?
diphosphate + TDP-glucose poor substrate Sorghum bicolor TTP + alpha-D-glucose 1-phosphate
-
r
additional information no activity with ADP-glucose Sorghum bicolor ?
-
?
additional information no activity with ATP, CTP, and GTP Sorghum bicolor ?
-
?
additional information no activity with TPP Sorghum bicolor ?
-
?
UTP + alpha-D-glucose 1-phosphate highly specific for UTP Sorghum bicolor diphosphate + UDP-glucose specific for UDP-glucose r
UTP + alpha-D-glucose 1-phosphate reverse reaction: highly specific for UDP-glucose Sorghum bicolor diphosphate + UDP-glucose specific for UDP-glucose r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Sorghum bicolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 9 pyrophosphorolysis Sorghum bicolor
8 9 UDP-glucose synthesis Sorghum bicolor

pH Range

pH Minimum pH Maximum Comment Organism
7 9 about 75% of maximal activity at pH 7.0 and maximal activity at pH 9.0 Sorghum bicolor

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.05
-
UDP-D-glucose pH 8.5, 37°C Sorghum bicolor
0.104
-
UTP pH 8.5, 37°C Sorghum bicolor