Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Sorghum bicolor | |
additional information | no activation by 3-phosphoglycerate | Sorghum bicolor | |
additional information | no activation by fructose 1,6-bisphosphate, fructose 6-phosphate, phosphoenolpyruvate or p-hydroxymandelonitrile-beta-D-glucopyranoside | Sorghum bicolor |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
alpha-D-glucose 1-phosphate | product inhibition | Sorghum bicolor | |
Co2+ | in excess of diphosphate-concentration, activates at lower concentrations | Sorghum bicolor | |
Mg-diphosphate | product inhibition | Sorghum bicolor | |
Mn2+ | in excess of diphosphate-concentration, activates at lower concentrations | Sorghum bicolor | |
UDP-glucose | product inhibition | Sorghum bicolor | |
UTP | product inhibition | Sorghum bicolor |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Sorghum bicolor | |
0.03 | - |
UTP | pH 8.5, 37°C | Sorghum bicolor | |
0.03 | 0.048 | alpha-D-glucose 1-phosphate | pH 8.5, 37°C | Sorghum bicolor | |
0.05 | 0.066 | UDP-glucose | - |
Sorghum bicolor | |
0.054 | - |
diphosphate | pH 8.5, 37°C | Sorghum bicolor |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | activation | Sorghum bicolor | |
Co2+ | Co2+ in excess of diphosphate-concentration inhibits | Sorghum bicolor | |
Mg2+ | requirement | Sorghum bicolor | |
Mg2+ | increasing MgSO4 concentrations shift the apparent equilibrium toward UDP-glucose synthesis | Sorghum bicolor | |
Mg2+ | Mg-diphosphate is the actual substrate | Sorghum bicolor | |
Mn2+ | activation | Sorghum bicolor | |
Mn2+ | UDP-glucose synthesis: Mn2+ in excess of diphosphate-concentration inhibits | Sorghum bicolor | |
Mn2+ | about 25% as effective as Mg2+ | Sorghum bicolor | |
additional information | requirement for divalent cations | Sorghum bicolor |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum bicolor | - |
cv. Rox Orange | - |
Purification (Comment) | Organism |
---|---|
- |
Sorghum bicolor |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose | sequential ordered bi bi reaction mechanism | Sorghum bicolor |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seedling | etiolated | Sorghum bicolor | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1200 | - |
purified enzyme | Sorghum bicolor |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + CDP-glucose | no activity | Sorghum bicolor | CTP + alpha-D-glucose 1-phosphate | - |
? | |
diphosphate + GDP-glucose | no activity | Sorghum bicolor | GTP + alpha-D-glucose 1-phosphate | - |
? | |
diphosphate + TDP-glucose | poor substrate | Sorghum bicolor | TTP + alpha-D-glucose 1-phosphate | - |
r | |
additional information | no activity with ADP-glucose | Sorghum bicolor | ? | - |
? | |
additional information | no activity with ATP, CTP, and GTP | Sorghum bicolor | ? | - |
? | |
additional information | no activity with TPP | Sorghum bicolor | ? | - |
? | |
UTP + alpha-D-glucose 1-phosphate | highly specific for UTP | Sorghum bicolor | diphosphate + UDP-glucose | specific for UDP-glucose | r | |
UTP + alpha-D-glucose 1-phosphate | reverse reaction: highly specific for UDP-glucose | Sorghum bicolor | diphosphate + UDP-glucose | specific for UDP-glucose | r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sorghum bicolor |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | pyrophosphorolysis | Sorghum bicolor |
8 | 9 | UDP-glucose synthesis | Sorghum bicolor |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | about 75% of maximal activity at pH 7.0 and maximal activity at pH 9.0 | Sorghum bicolor |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
UDP-D-glucose | pH 8.5, 37°C | Sorghum bicolor | |
0.104 | - |
UTP | pH 8.5, 37°C | Sorghum bicolor |