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Literature summary for 2.7.7.9 extracted from
- Pyrophosphorylases in Solanum tuberosum. IV. Purification, tissue localization, and physicochemical properties of UDP-glucose pyrophosphorylase (1993), Plant Physiol., 101, 1073-1080.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by 3-phosphoglycerate | Solanum tuberosum | |
| additional information | no activation by fructose 2,6-bisphosphate, UDPglucose synthesis | Solanum tuberosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-phosphoglycerate | no inhibition of UDP-glucose synthesis | Solanum tuberosum |  |
| D-fructose 2,6-bisphosphate | no inhibition of UDP-glucose synthesis | Solanum tuberosum | |
| D-fructose 6-phosphate | - |
Solanum tuberosum | |
| phosphate | - |
Solanum tuberosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics | Solanum tuberosum | |
| 0.055 | 0.08 | alpha-D-glucose 1-phosphate | at physiological substrate concentration, pH 8.5, 37°C | Solanum tuberosum | |
| 0.1 | 0.2 | UTP | at physiological substrate concentrations, pH 8.5, 37°C | Solanum tuberosum | |
| 0.11 | 0.13 | diphosphate | at physiological substrate concentration, pH 8.5, 37°C | Solanum tuberosum | |
| 0.12 | 0.18 | UDP-glucose | at physiological substrate concentrations, pH 8.5, 37°C | Solanum tuberosum | |
| 0.53 | - |
UTP | at substrate concentrations above 2 mM, pH 8.5, 37°C | Solanum tuberosum | |
| 0.68 | 1 | alpha-D-glucose 1-phosphate | at substrate concentrations above 2 mM, pH 8.5, 37°C | Solanum tuberosum | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Solanum tuberosum | |
| Mg2+ | 5 mM | Solanum tuberosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
gel filtration | Solanum tuberosum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Solanum tuberosum | - |
cv. Norchip | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 177fold to near homogeneity | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Solanum tuberosum | - |
| leaf | low level and activity | Solanum tuberosum | - |
| additional information | tissue distribution | Solanum tuberosum | - |
| root | low level and activity | Solanum tuberosum | - |
| stem | - |
Solanum tuberosum | - |
| stolon | - |
Solanum tuberosum | - |
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
activity in different tissues | Solanum tuberosum |
| 1099 | - |
purified enzyme | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | no activity with ADP-glucose | Solanum tuberosum | ? | - |
? | |
| additional information | no activity with ATP, CTP, and GTP | Solanum tuberosum | ? | - |
? | |
| UTP + alpha-D-glucose 1-phosphate | - |
Solanum tuberosum | diphosphate + UDP-glucose | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 53000, SDS-PAGE | Solanum tuberosum |
| More | multimeric forms may exist | Solanum tuberosum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UGPase | - |
Solanum tuberosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Solanum tuberosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Solanum tuberosum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9 | - |
Solanum tuberosum |
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