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Literature summary for 2.7.7.9 extracted from
- Purification of UDP-glucose pyrophosphorylase from germinated barley (malt) (1994), J. Biotechnol., 34, 157-163.
General Stability
| General Stability | Organism |
|---|---|
| bovine serum albumin stabilizes | Hordeum vulgare subsp. vulgare |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
gel filtration | Hordeum vulgare subsp. vulgare |
| 54000 | - |
1 * 54000, SDS-PAGE | Hordeum vulgare subsp. vulgare |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hordeum vulgare subsp. vulgare | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, 367fold | Hordeum vulgare subsp. vulgare |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | germinated, malt | Hordeum vulgare subsp. vulgare | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UTP + alpha-D-glucose 1-phosphate | - |
Hordeum vulgare subsp. vulgare | diphosphate + UDP-glucose | - |
r |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 54000, SDS-PAGE | Hordeum vulgare subsp. vulgare |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 60 | - |
Hordeum vulgare subsp. vulgare |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
30 h, stable at pH 8.0 with 0.1 mg/ml bovine serum albumin, without bovine serum albumin: t1/2: 7 h | Hordeum vulgare subsp. vulgare |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 7.5 | - |
Hordeum vulgare subsp. vulgare |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | 47 h, 25°C, 0.1 mg/ml bovine serum albumin, 5% loss of activity. Without bovine serum albumin: 80% or 60% loss of activity within 30 h at pH 6 or pH 6.5, respectively, t1/2: 30 h at pH 7.5 and pH 8.5 | Hordeum vulgare subsp. vulgare |
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Release 2023.1 (January 2023)
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