Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Bis-(3-aminopropyl)-amine | optimal polymerization activity requires presence, 2fold increase in activity at 30 mM | Nicotiana tabacum | |
dithiothreitol | similar activation as with 2-mercaptoethanol | Nicotiana tabacum | |
mercaptoethanol | 20 mM, 3fold activation | Nicotiana tabacum | |
NH4+ | maximal activation at 100 mM | Nicotiana tabacum | |
putrescine | optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM | Nicotiana tabacum | |
spermidine | optimal polymerization activity requires polyamines, 1.4fold increase in activity at 30 mM | Nicotiana tabacum | |
spermine | optimal polymerization activity requires polyamines, 2fold increase in activity at 30 mM | Nicotiana tabacum |
General Stability | Organism |
---|---|
insensitive to freezing | Nicotiana tabacum |
stable against several freezing and thawing cycles | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | polymerization reaction is not inhibited by diphosphate | Nicotiana tabacum | |
phosphate | 0.1 mM, complete inhibition of polymerization activity | Nicotiana tabacum | |
Poly(A) | inhibition of poly(U) synthesis | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
K+ | maximal activation at 200 mm | Nicotiana tabacum | |
Mg2+ | Mg2+ or Mn2+ required for activity, maximal activation at 1 mM Mg2+, inhibition above | Nicotiana tabacum | |
Mn2+ | Mg2+ or Mn2+ required for activity, maximal activation at 0.06 mM Mn2+, inhibition above | Nicotiana tabacum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
sedimentation analysis | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
L. var Samsun, healthy and TMV-infected leaves | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sephadex, enzyme from healthy and TMV-infected leaves | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0002 | - |
incorporation of UDP into polynucleotide | Nicotiana tabacum |
Storage Stability | Organism |
---|---|
-75°C, several months, no loss of activity | Nicotiana tabacum |
4°C, 1 week, 50% loss of activity | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
poly(A) + ADP | - |
Nicotiana tabacum | poly(A)+1 + phosphate | - |
r | |
RNAn + a nucleoside diphosphate | synthetic activity enhanced in presence of a primer | Nicotiana tabacum | RNAn+1 + phosphate | - |
? | |
RNAn + a nucleoside diphosphate | polymerization of ADP | Nicotiana tabacum | RNAn+1 + phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
60 min assay | Nicotiana tabacum |
35 | - |
10 min assay | Nicotiana tabacum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
polymerization of ADP | Nicotiana tabacum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7.6 | 8.8 | approx. 40% of maximal activity at pH 7.6, approx. 70% of maximal activity at pH 8.8 | Nicotiana tabacum |