Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | varying magnesium concentration affects both slippage and overall DNA synthesisof DNA polymerase PolB and PolD. There is almost no synthesis by PolB, PolD or their exo-forms at low magnesium concentrations (0.10.5 mM). Synthesis is also inhibited at 1520 mM. Parental molecules are readily detectable together with heteroduplex molecules at low to medium magnesium concentrations (15 mM) | Pyrococcus abyssi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus abyssi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | DNA polymerases PolB and PolD slip in vitro on a template that consists of single-stranded DNA (ssDNA) with a hairpin structure flanked by short direct repeats. Pyrococcus abyssi proliferating cell nuclear antigen increases replication fidelity of this template | Pyrococcus abyssi | ? | - |
? |