Literature summary for 2.7.7.7 extracted from

Schamber-Reis, B.L.; Nardelli, S.; Regis-Silva, C.G.; Campos, P.C.; Cerqueira, P.G.; Lima, S.A.; Franco, G.R.; Macedo, A.M.; Pena, S.D.; Cazaux, C.; Hoffmann, J.S.; Motta, M.C.; Schenkman, S.; Teixeira, S.M.; Machado, C.R.
DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions (2012), Mol. Biochem. Parasitol., 183, 122-131.

Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli as maltose-binding protein fusion protein	Trypanosoma cruzi

Protein Variants

Protein Variants	Comment	Organism
additional information	parasites overexpressing Tcpolbeta shows reduced levels of 7,8-dihydro-8-oxoguanine in kDNA and an increased survival after treatment with hydrogen peroxide when compared to control cells. The resistance is lost after treating Tcpolbeta overexpressors with methoxiamine, a potent base-excision repair inhibitor	Trypanosoma cruzi

Inhibitors

Inhibitors	Comment	Organism	Structure
3'-azido-2',3'-dideoxythymidine triphosphate	-	Trypanosoma cruzi

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
kinetoplast	Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes	Trypanosoma cruzi	20023	-
mitochondrial matrix	-	Trypanosoma cruzi	5759	-
additional information	subcellular localization of Tcpolbeta is restricted to antipodal sites in replicating cells, while in nonreplicative cultured trypomastigotes Tcpolbeta is dispersed inside its single ramified mitochondrion. The subcellular localization of Tcpolbeta follows the parasite cell cycle, overview	Trypanosoma cruzi	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	Trypanosoma cruzi	-	diphosphate + DNAn+1	-	?

Organism

Organism	UniProt	Comment	Textmining
Trypanosoma cruzi	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
amastigote	Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes	Trypanosoma cruzi	-
epimastigote	Trypanosoma cruzi DNA polymerase beta is restricted to the antipodal sites of kDNA in replicative epimastigote and amastigote developmental stages, being strictly localized to kDNA antipodal sites between G1/S and early G2 phase in replicative epimastigotes	Trypanosoma cruzi	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
deoxynucleoside triphosphate + DNAn	-	Trypanosoma cruzi	diphosphate + DNAn+1	-	?
dTTP + DNAn	-	Trypanosoma cruzi	diphosphate + DNAn+1	-	?

Synonyms

Synonyms	Comment	Organism
DNA polymerase beta	-	Trypanosoma cruzi
polbeta	-	Trypanosoma cruzi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assya at	Trypanosoma cruzi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Trypanosoma cruzi

General Information

General Information	Comment	Organism
physiological function	DNA polymerase beta from Trypanosoma cruzi is involved in kinetoplast DNA replication and repair of oxidative lesions, Tcpolbeta is involved in oxidative stress response, Tcpolbeta overexpression improves epimastigote survival in presence of H2O2	Trypanosoma cruzi

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)