Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pyrococcus horikoshii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | deletion of the COOH-terminal zinc finger region (12891308) does not abolish the subunit interaction. Instead, while the polymerization of the deletion mutants remains, the 3'-5' exonuclease is completely inactivated. Deletion of DP1Pho(1200) increases the exonuclease and DNA binding activities of PolDPho. Adding DP1Pho(1200) to the truncated protein suppresses the elevated exonuclease activity | Pyrococcus horikoshii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | Pyrococcus horikoshii | the enzyme plays an essential role in DNA replication, repair, and recombination | diphosphate + DNAn+1 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus horikoshii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrococcus horikoshii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | the enzyme plays an essential role in DNA replication, repair, and recombination | Pyrococcus horikoshii | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | the carboxyl-terminal (12551332) of the large subunit (DP2Pho) and two regions, the 201260 and 599622, of the small subunit (DP1Pho) are critical for the complex formation, and probable subunit interaction of PolDPho. The amino-terminal (1300) of DP2Pho is essential for the folding of PolDPho and is likely the oligomerization domain of PolDPho | Pyrococcus horikoshii | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PolDPho | - |
Pyrococcus horikoshii |
General Information | Comment | Organism |
---|---|---|
physiological function | the enzyme plays an essential role in DNA replication, repair, and recombination | Pyrococcus horikoshii |