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Literature summary for 2.7.7.7 extracted from

  • Shen, Y.; Tang, X.F.; Matsui, I.
    Subunit interaction and regulation of activity through terminal domains of the family D DNA polymerase from Pyrococcus horikoshii (2003), J. Biol. Chem., 278, 21247-21257.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus horikoshii

Protein Variants

Protein Variants Comment Organism
additional information deletion of the COOH-terminal zinc finger region (1289–1308) does not abolish the subunit interaction. Instead, while the polymerization of the deletion mutants remains, the 3'-5' exonuclease is completely inactivated. Deletion of DP1Pho(1–200) increases the exonuclease and DNA binding activities of PolDPho. Adding DP1Pho(1–200) to the truncated protein suppresses the elevated exonuclease activity Pyrococcus horikoshii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Pyrococcus horikoshii the enzyme plays an essential role in DNA replication, repair, and recombination diphosphate + DNAn+1
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?

Organism

Organism UniProt Comment Textmining
Pyrococcus horikoshii
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-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the enzyme plays an essential role in DNA replication, repair, and recombination Pyrococcus horikoshii diphosphate + DNAn+1
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?
deoxynucleoside triphosphate + DNAn the carboxyl-terminal (1255–1332) of the large subunit (DP2Pho) and two regions, the 201–260 and 599–622, of the small subunit (DP1Pho) are critical for the complex formation, and probable subunit interaction of PolDPho. The amino-terminal (1–300) of DP2Pho is essential for the folding of PolDPho and is likely the oligomerization domain of PolDPho Pyrococcus horikoshii diphosphate + DNAn+1
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?

Synonyms

Synonyms Comment Organism
PolDPho
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Pyrococcus horikoshii

General Information

General Information Comment Organism
physiological function the enzyme plays an essential role in DNA replication, repair, and recombination Pyrococcus horikoshii