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Literature summary for 2.7.7.7 extracted from
- Characterization and PCR application of a new high-fidelity DNA polymerase from Thermococcus waiotapuensis (2012), Enzyme Microb. Technol., 51, 334-341.
Application
| Application | Comment | Organism |
|---|---|---|
| molecular biology | the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay | Thermococcus waiotapuensis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Thermococcus waiotapuensis |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | activates | Thermococcus waiotapuensis |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
x * 90000, SDS-PAGE | Thermococcus waiotapuensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus waiotapuensis | H9CW54 | - |
- |
| Thermococcus waiotapuensis DSM 12768 | H9CW54 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermococcus waiotapuensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme also posseses 3'->5' exonuclease activity | Thermococcus waiotapuensis | ? | - |
? | |
| additional information | the enzyme also posseses 3'->5' exonuclease activity | Thermococcus waiotapuensis DSM 12768 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 90000, SDS-PAGE | Thermococcus waiotapuensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Thermococcus waiotapuensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 85 | 60°C: about 65% of maximal activity, 85°C: about 75% of maximal activity | Thermococcus waiotapuensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 99 | - |
half-life: 4 h | Thermococcus waiotapuensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Thermococcus waiotapuensis |
| 7.5 | - |
assay at | Thermococcus waiotapuensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 6.5 | pH 5.5: about 50% of maximal activity, pH 6.5: about 50% of maximal activity | Thermococcus waiotapuensis |
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