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Literature summary for 2.7.7.7 extracted from

  • Lee, J.I.; Kim, Y.J.; Bae, H.; Cho, S.S.; Lee, J.H.; Kwon, S.T.
    Biochemical properties and PCR performance of a family B DNA polymerase from hyperthermophilic Euryarchaeon Thermococcus peptonophilus (2010), Appl. Biochem. Biotechnol., 160, 1585-1599.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermococcus peptonophilus

Protein Variants

Protein Variants Comment Organism
molecular biology the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay Thermococcus peptonophilus

Metals/Ions

Metals/Ions Comment Organism Structure
KCl optimal concentration of 80 mM Thermococcus peptonophilus
Mg2+ highly dependent on MgCl2, with maximal activity at 14 mM MgCl2 and no detectable activity in the absence of MgCl2 Thermococcus peptonophilus

Organism

Organism UniProt Comment Textmining
Thermococcus peptonophilus
-
-
-
Thermococcus peptonophilus DSM 10343
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermococcus peptonophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Thermococcus peptonophilus diphosphate + DNAn+1
-
?
deoxynucleoside triphosphate + DNAn
-
Thermococcus peptonophilus DSM 10343 diphosphate + DNAn+1
-
?
additional information the DNA polymerase possesses a 3'->5' exonuclease activity Thermococcus peptonophilus ?
-
?
additional information the DNA polymerase possesses a 3'->5' exonuclease activity Thermococcus peptonophilus DSM 10343 ?
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
Thermococcus peptonophilus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
65 85 65°C: about 70% of maximal activity, 85°C: about 50% of maximal activity Thermococcus peptonophilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
half-life: 4 h Thermococcus peptonophilus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Thermococcus peptonophilus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 pH 6.5: about 60% of maximal activity, pH 7.5: about 89% of maximal activity Thermococcus peptonophilus