Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus peptonophilus |
Protein Variants | Comment | Organism |
---|---|---|
molecular biology | the high fidelty of the enzyme is suitable fo polymerase chain reaction (PCR), which requires accurate DNA amplification for gene cloning and diagnostic assay | Thermococcus peptonophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | optimal concentration of 80 mM | Thermococcus peptonophilus | |
Mg2+ | highly dependent on MgCl2, with maximal activity at 14 mM MgCl2 and no detectable activity in the absence of MgCl2 | Thermococcus peptonophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermococcus peptonophilus | - |
- |
- |
Thermococcus peptonophilus DSM 10343 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus peptonophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | - |
Thermococcus peptonophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | - |
Thermococcus peptonophilus DSM 10343 | diphosphate + DNAn+1 | - |
? | |
additional information | the DNA polymerase possesses a 3'->5' exonuclease activity | Thermococcus peptonophilus | ? | - |
? | |
additional information | the DNA polymerase possesses a 3'->5' exonuclease activity | Thermococcus peptonophilus DSM 10343 | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
- |
Thermococcus peptonophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 85 | 65°C: about 70% of maximal activity, 85°C: about 50% of maximal activity | Thermococcus peptonophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
half-life: 4 h | Thermococcus peptonophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Thermococcus peptonophilus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | pH 6.5: about 60% of maximal activity, pH 7.5: about 89% of maximal activity | Thermococcus peptonophilus |