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Literature summary for 2.7.7.7 extracted from

  • Sharma, A.; Nair, D.T.
    Cloning, expression, purification, crystallization and preliminary crystallographic analysis of MsDpo4: a Y-family DNA polymerase from Mycobacterium smegmatis (2011), Acta Crystallogr. Sect. F, 67, 812-816.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Cloned(Commentary)

Cloned (Comment) Organism
Rv1537 and Rv3056, expression of GST-tagged MsDpo4 in Escherichia coli strain C41(DE3) Mycolicibacterium smegmatis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified native and selenomethionine-labeled MsDpo4, hanging drop vapour diffusion method, 1 mM protein is mmixed with 2.4 M NaCl, and 0.1 M sodium/potassium phosphate, pH 6.4, 72 h, 25°C, optimization of crystallization and cryoprotection method, X-ray diffraction structure determination and analysis at 26 A resolution, molecular replacement Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39146
-
 x * 39146, purified recombinant detagged enzyme, mass spectrometry Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0QR77 two family Y DNA polymerases Rv1537 and Rv3056
-

Purification (Commentary)

Purification (Comment) Organism
GST-tagged MsDpo4 from Escherichia coli strain C41(DE3) by glutathione affinity chromatography, the tag is cleaved off Mycolicibacterium smegmatis

Subunits

Subunits Comment Organism
? x * 39146, purified recombinant detagged enzyme, mass spectrometry Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
DNA polymerase IV
-
 Mycolicibacterium smegmatis
MsDpo4
-
 Mycolicibacterium smegmatis
Rv1537
-
 Mycolicibacterium smegmatis
Rv3056
-
 Mycolicibacterium smegmatis

Expression

Organism Comment Expression
Mycolicibacterium smegmatis the expression of Rv1537 and Rv3056 is not upregulated under conditions of stress additional information

General Information

General Information Comment Organism
physiological function error-prone DNA polymerases belonging to the Y-family in mycobacteria do not participate in adaptive mutagenesis, in contrast to the representatives of theis faamily in other prokaryotes. Escherichia coli gene dinB homologues in mycobacteria code for nonfunctional molecules that are devoid of enzyme activity Mycolicibacterium smegmatis