Cloned (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
F12A | mutant ribonucleoside triphosphates almost as efficiently as deoxyribonucleoside triphosphates, and, unlike analogous mutants in other polymerase families, shows no barrier to adding multiple ribonucleotides, suggesting that Dbh can readily accommodate a DNA/RNA duplex product | Saccharolobus solfataricus |
F12A | GTP incorporation by the wild-type enzyme is about 1000fold slower than dGTP incorporation. The rate of GTP incorporation by the mutant enzyme F12A Dbh is 2-3fold slower than incorporation of dGTP. When making a deletion error, ribonucleotide discrimination by wild-type and F12A Dbh is the same as in normal DNA synthesis | Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Saccharolobus solfataricus | P96022 | - |
- |
Purification (Comment) | Organism |
---|---|
wild-type and F12A mutant | Saccharolobus solfataricus |
wild-type and mutant enzyme F12A | Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | ribonucleotide discrimination by the DinB homolog (Dbh) DNA polymerase is as stringent as in other polymerases. When making a deletion error, ribonucleotide discrimination by wild-type and F12A Dbh is the same as in normal DNA synthesis | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | GTP incorporation by the wild-type enzyme is about 1000fold slower than dGTP incorporation. The rate of GTP incorporation by the mutant enzyme F12A Dbh is 2-3fold slower than incorporation of dGTP. The enzyme makes single-base deletion errors at high frequency in particular sequence contexts | Saccharolobus solfataricus | diphosphate + DNAn+1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Dbh DNA polymerase | - |
Saccharolobus solfataricus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme | Saccharolobus solfataricus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state kinetic constants for dNTP and rNTP incorporation by wild-type and F12A mutant enzyme | Saccharolobus solfataricus |