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Literature summary for 2.7.7.7 extracted from

  • Klimczak, L.J.; Grummt, F.; Burger, K.J.
    Purification and characterization of DNA polymerase from the archaebacterium Sulfolobus acidocaldarius (1985), Nucleic Acids Res., 13, 5269-5282.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.7.7

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM Sulfolobus acidocaldarius
KCl 100 mM, 80% inhibition Sulfolobus acidocaldarius
Mg2+ above 2 mM Sulfolobus acidocaldarius
additional information no inhibition by 0.02 mg/ml aphidicolin. The enzyme is inhibited by dideoxy- and arabinosine-analogs and SH-blocking agents Sulfolobus acidocaldarius

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ the enzyme requires an extremely low concentration of Mg+2 cations for activity. A broad plateau is observed between 0.1 and 2 mM MgCl2. Concentrations above 2 mM are inhibitory. 20% of the activity of the plateau value is still observed even if no MgCl2 is added to the assay Sulfolobus acidocaldarius

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
100000
-
 1 * 100000, SDS-PAGE Sulfolobus acidocaldarius
108000
-
 gel filtration Sulfolobus acidocaldarius

Organism

Organism UniProt Comment Textmining
Sulfolobus acidocaldarius P95690
-
-
Sulfolobus acidocaldarius DSM 639 P95690
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Sulfolobus acidocaldarius

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
178
-
 pH 8.0, 65°C, incorporation of TTP Sulfolobus acidocaldarius

Storage Stability

Storage Stability Organism
-20°C, in the presence of 20% glycerol, no substantial loss of activity after several weeks Sulfolobus acidocaldarius

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn the activated herring sperm DNA is an optimal template and gives 3times higher activity than that obtained with poly(dA)/oligo(dT). The DNA polymerase can not use templates without primer (poly(dA) and poly(dT)), even when appropriate priming ribonucleotides (UTP or ATP, respectively) are supplied. It does not accept a polyribonucleotide template (poly(rA):oligo(dT)) Sulfolobus acidocaldarius diphosphate + DNAn+1
-
 ?
deoxynucleoside triphosphate + DNAn the activated herring sperm DNA is an optimal template and gives 3times higher activity than that obtained with poly(dA)/oligo(dT). The DNA polymerase can not use templates without primer (poly(dA) and poly(dT)), even when appropriate priming ribonucleotides (UTP or ATP, respectively) are supplied. It does not accept a polyribonucleotide template (poly(rA):oligo(dT)) Sulfolobus acidocaldarius DSM 639 diphosphate + DNAn+1
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE Sulfolobus acidocaldarius

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
 Sulfolobus acidocaldarius

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
37 65 activity at 65°C (optimum) is 20fold higher than at 37°C. A rapid 10fold increase occurs at 70-80°C Sulfolobus acidocaldarius

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 maximal activity in 50 mM potassium phosphate buffer Sulfolobus acidocaldarius
8
-
 assay at Sulfolobus acidocaldarius
8
-
 maximal activity in 50 mM Tris-HCl buffer Sulfolobus acidocaldarius

pH Range

pH Minimum pH Maximum Comment Organism
5.5 8 pH 5.5: about 70% of maximal activity, pH 8.0: about 20% of maximal activity, maximal activity in 50 mM potassium phosphate buffer Sulfolobus acidocaldarius
7 9.5 pH 7.0: about 70% of maximal activity, pH 9.5: about 70% of maximal activity, in 50 mM Tris-HCl buffer Sulfolobus acidocaldarius