Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Thermus thermophilus |
Protein Variants | Comment | Organism |
---|---|---|
D349A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
D529A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
E413A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
H344A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
H374A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
H440A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
H468A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
H531A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
Q342A | the Mg2+-dependent DNA polymerase activity of the mutant is almost the same as that of wild type enzyme | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
DNAn | in the presence of dNTPs | Thermus thermophilus | |
0.0017 | - |
DNAn | in the absence of dNTPs | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | PolX has Mg2+-dependent 5'-3' DNA polymerase activity | Thermus thermophilus | |
additional information | the PolX catalytic domain exhibits no polymerase activity with 5 mM Zn2+, Ni2+, Ca2+ or Co2+, or without a metal ion | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
64000 | - |
estimated from SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Thermus thermophilus | the catalytic efficiency of PolX is almost the same with and without dNTPs, whereas that of the domain mixture increases on the addition of dNTPs | ? | - |
? | |
additional information | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | the catalytic efficiency of PolX is almost the same with and without dNTPs, whereas that of the domain mixture increases on the addition of dNTPs | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | - |
- |
- |
Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Toyopearl phenyl-650M column chroamtography, Super Q-650M column chromatography, hydroxyapatite column chromatography, Superdex 200 gel filtration, His-bind resin column chromatography, Toyopearl butyl column chromatography, and Superdex 75 gel filtration | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
deoxynucleoside triphosphate + DNAn | in the presence of Mg2+ or Mn2+, the POLX catalytic domain inserts dIMP, IMP and 8-oxo-dGMP opposite deoxycytosine as well as dGMP and GMP, PolX likely prefers deoxyguanine to deoxythymidine | Thermus thermophilus | diphosphate + DNAn+1 | - |
? | |
deoxynucleoside triphosphate + DNAn | in the presence of Mg2+ or Mn2+, the POLX catalytic domain inserts dIMP, IMP and 8-oxo-dGMP opposite deoxycytosine as well as dGMP and GMP, PolX likely prefers deoxyguanine to deoxythymidine | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | diphosphate + DNAn+1 | - |
? | |
additional information | the catalytic efficiency of PolX is almost the same with and without dNTPs, whereas that of the domain mixture increases on the addition of dNTPs | Thermus thermophilus | ? | - |
? | |
additional information | the catalytic efficiency of PolX is almost the same with and without dNTPs, whereas that of the domain mixture increases on the addition of dNTPs | Thermus thermophilus HB8 / ATCC 27634 / DSM 579 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DNA polymerase X | - |
Thermus thermophilus |
Pol I | - |
Thermus thermophilus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
28 | - |
DNAn | in the absence of dNTPs | Thermus thermophilus | |
38 | - |
DNAn | in the presence of dNTPs | Thermus thermophilus |