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Literature summary for 2.7.7.7 extracted from

  • Kim, S.W.; Kim, D.U.; Kim, J.K.; Kang, L.W.; Cho, H.S.
    Crystal structure of Pfu, the high fidelity DNA polymerase from Pyrococcus furiosus (2008), Int. J. Biol. Macromol., 42, 356-361.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
using 0.2 M ammonium sulfate, 0.1 M Na-cacodylate (pH 6.5), 5 mM dithiothreitol, 50 mM MnCl2, and 15% (w/v) PEG 8000 Pyrococcus furiosus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Pyrococcus furiosus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus P61875
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE Sephacel gel filtration and HiTrap heparin column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Pyrococcus furiosus diphosphate + DNAn+1
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase
-
Pyrococcus furiosus
Pfu a family B DNA polymerase Pyrococcus furiosus