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Literature summary for 2.7.7.7 extracted from
- Characterization of intein homing endonuclease encoded in the DNA polymerase gene of Thermococcus marinus (2009), FEMS Microbiol. Lett., 297, 180-188.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli Rosetta(DE3) cells | Thermococcus marinus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 90000 | - |
mature DNA polymerase, SDS-PAGE | Thermococcus marinus |
| 152000 | - |
precursor form of DNA polymerase containing 62 kDa intein, SDS-PAGE | Thermococcus marinus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Thermococcus marinus | the DNA polymerase gene of Thermococcus marinus contains an intein inserted at the pol-b site | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermococcus marinus | C7AIP4 | strain DSM 15227 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| HiTrap heparin column chromatography, gel filtration | Thermococcus marinus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| deoxynucleoside triphosphate + DNAn | - |
Thermococcus marinus | diphosphate + DNAn+1 | - |
? | |
| additional information | the DNA polymerase gene of Thermococcus marinus contains an intein inserted at the pol-b site | Thermococcus marinus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| DNA polymerase | - |
Thermococcus marinus |
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Release 2023.1 (January 2023)
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