Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.7.7 extracted from

  • Fiala, K.A.; Sherrer, S.M.; Brown, J.A.; Suo, Z.
    Mechanistic consequences of temperature on DNA polymerization catalyzed by a Y-family DNA polymerase (2008), Nucleic Acids Res., 36, 1990-2001.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information pre-steady state kinetics, and biphasic kinetics of nucleotide incorporation at 56°C Saccharolobus solfataricus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
deoxynucleoside triphosphate + DNAn Saccharolobus solfataricus
-
diphosphate + DNAn+1
-
?

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
a 2'-deoxyribonucleoside 5'-triphosphate + DNAn = diphosphate + DNAn+1 reaction mechanism Saccharolobus solfataricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
deoxynucleoside triphosphate + DNAn
-
Saccharolobus solfataricus diphosphate + DNAn+1
-
?
additional information Dpo4 utilizes an induced-fit mechanism to select correct incoming nucleotides at 37°C, overview Saccharolobus solfataricus ?
-
?

Synonyms

Synonyms Comment Organism
DNA polymerase
-
Saccharolobus solfataricus
Dpo4
-
Saccharolobus solfataricus
More the enzyme belongs to the DNA polymerase Y-family Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharolobus solfataricus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2 80 the incorporation rate for correct nucleotides increases by 18900fold from 2°C to 56°C, the fidelity of Dpo4 at 80°C is similar to that determined at 56°C Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Saccharolobus solfataricus