Cloned (Comment) | Organism |
---|---|
overexpression of RB69 single-stranded DNA binding protein-DNA polymerase fusion | Escherichia phage RB69 |
Crystallization (Comment) | Organism |
---|---|
the crystal structure of this fusion protein is solved by a combination of multiwavelength anomalous diffraction and molecular replacement to 3.2 A resolution and shows that RB69 single-stranded DNA binding protein is positioned proximal to the N-terminal domain of RB69 DNA polymerase near the template strand channel | Escherichia phage RB69 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fusion of RB69 DNA polymerase with its cognate single-stranded DNA binding protein via a short six amino acid linker increases affinity for primer-template DNA by 6fold and subsequently increases processivity by 7fold while maintaining fidelity | Escherichia phage RB69 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia phage RB69 | - |
- |
- |